ID   Y3131_MYCTO             Reviewed;         344 AA.
AC   P9WIZ6; L0TD88; P95195; Q7D627;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   07-JAN-2015, sequence version 2.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Putative NAD(P)H nitroreductase MT3217 {ECO:0000305};
GN   OrderedLocusNames=MT3217;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
CC   -!- FUNCTION: Stimulates pro-inflammatory cytokine expression via TLR2
CC       signaling pathway. Activation of TLR2 results in the phosphorylation
CC       and activation of NF-kappa-B. Also induces TLR2 expression. May
CC       influence the innate immune responses to facilitate the survival of
CC       M.tuberculosis in the granulomatous microenvironment.
CC       {ECO:0000250|UniProtKB:P9WIZ7}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000305};
CC   -!- SUBUNIT: Interacts with human TLR2. {ECO:0000250|UniProtKB:P9WIZ7}.
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).
CC       {ECO:0000269|PubMed:12953092}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47555.1; -; Genomic_DNA.
DR   PIR; D70645; D70645.
DR   AlphaFoldDB; P9WIZ6; -.
DR   SMR; P9WIZ6; -.
DR   EnsemblBacteria; AAK47555; AAK47555; MT3217.
DR   KEGG; mtc:MT3217; -.
DR   HOGENOM; CLU_051479_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.109.10; -; 2.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   2: Evidence at transcript level;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase; Virulence.
FT   CHAIN           1..344
FT                   /note="Putative NAD(P)H nitroreductase MT3217"
FT                   /id="PRO_0000427918"
FT   BINDING         40..44
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255"
FT   BINDING         326
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   344 AA;  37049 MW;  6ACAD2A4C05DCB49 CRC64;
     MTAAVDGKGP AAMNTHFPDA ETVRTVLTLA VRAPSIHNTQ PWRWRVCPTS LELFSRPDMQ
     LRSTDPDGRE LILSCGVALH HCVVALASLG WQAKVNRFPD PKDRCHLATI GVQPLVPDQA
     DVALAAAIPR RRTDRRAYSC WPVPGGDIAL MAARAARGGV MLRQVSALDR MKAIVAQAVL
     DHVTDEEYLR ELTIWSGRYG SVAGVPARNE PPSDPSAPIP GRLFAGPGLS QPSDVLPADD
     GAAILALGTE TDDRLARLRA GEAASIVLLT ATAMGLACCP ITEPLEIAKT RDAVRAEVFG
     AGGYPQMLLR VGWAPINADP LPPTPRRELS QVVEWPEELL RQRC