Y3154_MYCBO
ID Y3154_MYCBO Reviewed; 463 AA.
AC P0A651; A0A1R3Y384; O07035; X2BMP4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Putative diacyglycerol O-acyltransferase Mb3154c;
DE EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE AltName: Full=Putative triacylglycerol synthase Mb3154c;
GN OrderedLocusNames=BQ2027_MB3154C;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIU01780.1; -; Genomic_DNA.
DR RefSeq; NP_856799.1; NC_002945.3.
DR RefSeq; WP_003899932.1; NC_002945.4.
DR AlphaFoldDB; P0A651; -.
DR SMR; P0A651; -.
DR EnsemblBacteria; SIU01780; SIU01780; BQ2027_MB3154C.
DR PATRIC; fig|233413.5.peg.3469; -.
DR OMA; RWAMLTK; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; PTHR31650; 1.
DR Pfam; PF03007; WES_acyltransf; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW Transferase.
FT CHAIN 1..463
FT /note="Putative diacyglycerol O-acyltransferase Mb3154c"
FT /id="PRO_0000222917"
FT ACT_SITE 137
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 463 AA; 50721 MW; 48CC60A8670F1D6B CRC64;
MNHLTTLDAG FLKAEDVDRH VSLAIGALAV IEGPAPDQEA FLSSLAQRLR PCTRFGQRLR
LRPFDLGAPK WVDDPDFDLG RHVWRIALPR PGNEDQLFEL IADLMARRLD RGRPLWEVWV
IEGLADSKWA ILTKLHHCMA DGIAATHLLA GLSDESMSDS FASNIHTTMQ SQSASVRRGG
FRVNPSEALT ASTAVMAGIV RAAKGASEIA AGVLSPAASS LNGPISDLRR YSAAKVPLAD
VEQVCRKFDV TINDVALAAI TESYRNVLIQ RGERPRFDSL RTLVPVSTRS NSALSKTDNR
VSLMLPNLPV DQENPLQRLR IVHSRLTRAK AGGQRQFGNT LMAIANRLPF PMTAWAVGLL
MRLPQRGVVT VATNVPGPRR PLQIMGRRVL DLYPVSPIAM QLRTSVAMLS YADDLYFGIL
ADYDVVADAG QLARGIEDAV ARLVAISKRR KVTRRRGALS LVV
