ID   Y3189_MYCTU             Reviewed;         206 AA.
AC   O53335; F2GJP4; I6YBF5; Q7D5Z1;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Probable NAD(+) phosphorylase Rv3189 {ECO:0000250|UniProtKB:P9WLP9};
DE            EC=2.4.2.- {ECO:0000250|UniProtKB:P9WLP9};
DE   AltName: Full=Mycobacterial cidal toxin Rv3189 {ECO:0000250|UniProtKB:P9WLP9};
GN   OrderedLocusNames=Rv3189;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2] {ECO:0007744|PubMed:21969609}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   DISCUSSION OF POSSIBLE FUNCTION.
RC   STRAIN=H37Rv;
RX   PubMed=30598453; DOI=10.1073/pnas.1814633116;
RA   Piscotta F.J., Jeffrey P.D., Link A.J.;
RT   "ParST is a widespread toxin-antitoxin module that targets nucleotide
RT   metabolism.";
RL   Proc. Natl. Acad. Sci. U.S.A. 116:826-834(2019).
CC   -!- FUNCTION: Probable toxic component of a type II toxin-antitoxin (TA)
CC       system. Degrades NAD(+) by phosphorolysis. Neutralized by its cognate
CC       antitoxin Rv3188. {ECO:0000250|UniProtKB:P9WLP9,
CC       ECO:0000305|PubMed:30598453}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + phosphate = ADP-beta-D-ribose 1''-phosphate + H(+) +
CC         nicotinamide; Xref=Rhea:RHEA:20788, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17154, ChEBI:CHEBI:43474, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:58753; Evidence={ECO:0000250|UniProtKB:P9WLP9};
CC   -!- SUBUNIT: Forms a heterotetramer with cognate antitoxin Rv3188.
CC       {ECO:0000250|UniProtKB:P9WLP9}.
CC   -!- SIMILARITY: Belongs to the MbcT/ParT/Res family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46000.1; -; Genomic_DNA.
DR   RefSeq; NP_217705.1; NC_000962.3.
DR   RefSeq; WP_003899957.1; NZ_NVQJ01000003.1.
DR   AlphaFoldDB; O53335; -.
DR   SMR; O53335; -.
DR   STRING; 83332.Rv3189; -.
DR   PaxDb; O53335; -.
DR   DNASU; 888034; -.
DR   GeneID; 888034; -.
DR   KEGG; mtu:Rv3189; -.
DR   PATRIC; fig|83332.111.peg.3553; -.
DR   TubercuList; Rv3189; -.
DR   OMA; NREQGAW; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR014914; RES_dom.
DR   Pfam; PF08808; RES; 1.
DR   SMART; SM00953; RES; 1.
PE   1: Evidence at protein level;
KW   NAD; Nucleotidyltransferase; Reference proteome; Toxin-antitoxin system;
KW   Transferase.
FT   CHAIN           1..206
FT                   /note="Probable NAD(+) phosphorylase Rv3189"
FT                   /id="PRO_0000448609"
SQ   SEQUENCE   206 AA;  22944 MW;  E487703890AF144A CRC64;
     MKLADAIATA PRRTLKGTYW HQGPTRHPVT SCADPARGPG RYHRTGEPGV WYASNKEQGA
     WAELFRHFVD DGVDPFEVRR RVGRVAVTLQ VLDLTDERTR SHLGVDETDL LSDDYTTTQA
     IAAARDANFD AVLAPAAALP GCQTLAVFVH ALPNIEPERS EVRQPPPRLA NLLPLIRPHE
     HMPDSVRRLL ATLTRAGAEA IRRRRR