ID   Y321_RICBR              Reviewed;         229 AA.
AC   Q1RJR2;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase RBE_0321;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=RBE_0321;
OS   Rickettsia bellii (strain RML369-C).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; belli group.
OX   NCBI_TaxID=336407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RML369-C;
RX   PubMed=16703114; DOI=10.1371/journal.pgen.0020076;
RA   Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C.,
RA   Fournier P.-E., Claverie J.-M., Raoult D.;
RT   "Genome sequence of Rickettsia bellii illuminates the role of amoebae in
RT   gene exchanges between intracellular pathogens.";
RL   PLoS Genet. 2:733-744(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; CP000087; ABE04402.1; -; Genomic_DNA.
DR   RefSeq; WP_011477013.1; NC_007940.1.
DR   AlphaFoldDB; Q1RJR2; -.
DR   SMR; Q1RJR2; -.
DR   STRING; 336407.RBE_0321; -.
DR   EnsemblBacteria; ABE04402; ABE04402; RBE_0321.
DR   KEGG; rbe:RBE_0321; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_0_5; -.
DR   OMA; SMEFAPF; -.
DR   OrthoDB; 1037615at2; -.
DR   Proteomes; UP000001951; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding.
FT   CHAIN           1..229
FT                   /note="Uncharacterized RNA pseudouridine synthase RBE_0321"
FT                   /id="PRO_0000294475"
FT   DOMAIN          2..69
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   229 AA;  26222 MW;  1864751558205D85 CRC64;
     MQRLAKLISN AGICSRRDAE KLILDGQVKV NGIIVTSPAT NVDTDNQVEV SGSLITSSQK
     SRLWIYYKPV GLITTHKDPL SRKTVFEQLT GLPRVISIGR LDLNSEGLLL LTNNGDLARQ
     FELPSSKLKR VYHVRAYGNP DPLLKSNYKN LEIDGIFYNP QSIKLLRKNS TNSWLEVILF
     EGKNREIRRI FEHFGLKVNK LIRTEYGDFK LDNLKPNEYR EVTKKLYKC