ID   Y3226_LACPL             Reviewed;         481 AA.
AC   Q88SY9; F9UTC9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Uncharacterized RNA methyltransferase lp_3226;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=lp_3226;
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AL935263; CCC80247.1; -; Genomic_DNA.
DR   RefSeq; WP_003642454.1; NC_004567.2.
DR   RefSeq; YP_004890761.1; NC_004567.2.
DR   AlphaFoldDB; Q88SY9; -.
DR   SMR; Q88SY9; -.
DR   STRING; 220668.lp_3226; -.
DR   PRIDE; Q88SY9; -.
DR   EnsemblBacteria; CCC80247; CCC80247; lp_3226.
DR   GeneID; 57026510; -.
DR   KEGG; lpl:lp_3226; -.
DR   PATRIC; fig|220668.9.peg.2695; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_1_9; -.
DR   OMA; YCGVGGF; -.
DR   PhylomeDB; Q88SY9; -.
DR   BioCyc; LPLA220668:G1GW0-2735-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..481
FT                   /note="Uncharacterized RNA methyltransferase lp_3226"
FT                   /id="PRO_0000161990"
FT   DOMAIN          29..87
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        438
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         313
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         342
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         363
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         411
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   481 AA;  53727 MW;  DC22FCE3311948A8 CRC64;
     MPQSNHYSHQ SRSHNDRRRQ QPDEKVQATV NIGQRFPLTI RRLGINGEGI GYYKHVITFV
     KGALPEEVVV AEVTAVHPRY LEAKIRSIRK PSPDRVDPRD AYAGEVGGFE LEHLDYPAQL
     AFKQDLIRQA LEKYRPAGYR HYDVRPTIGM TNPYEYRNKA QFQVRLIDGH VAAGLYKENS
     HDLVDLPTCS VQMPATMTVM RQVVAWLEEL QVPIYDEEHN SGIVKTIVVR EAAATGEIQL
     VFITNTPKLP KKHQLLMKIA EKLPMVVSVM QNINAGKTSL IWGDQTTLLA GKPTITEELD
     GLVFDLSARA FFQLNPQQTK KLYRLAREAL NLAPNETLVD AYSGVGTIGL SLADVAKEVR
     GMDTIPAAVA DANANAQRNQ ITNAHYEVGE AEVLLPQWLA SGFAPDAMVV DPPRTGLDNV
     LIDAILQSAP EKLVYISCNP STLAQDLQAL TRGYQVDYIQ SIDMFPQTAR CEAVVRFTKR
     H