ID   Y3240_PSEAE             Reviewed;         285 AA.
AC   Q9HZ00;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Putative quercetin 2,3-dioxygenase PA3240;
DE            Short=Putative quercetinase;
DE            EC=1.13.11.24;
DE   AltName: Full=Pirin-like protein PA3240;
GN   OrderedLocusNames=PA3240;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Putative quercetin 2,3-dioxygenase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6-
CC         dihydroxybenzoate + CO; Xref=Rhea:RHEA:15381, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17245, ChEBI:CHEBI:57628, ChEBI:CHEBI:57694;
CC         EC=1.13.11.24;
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 divalent metal cation. {ECO:0000250};
CC   -!- PATHWAY: Flavonoid metabolism; quercetin degradation.
CC   -!- SIMILARITY: Belongs to the pirin family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG06628.1; -; Genomic_DNA.
DR   PIR; B83239; B83239.
DR   RefSeq; NP_251930.1; NC_002516.2.
DR   RefSeq; WP_003114805.1; NZ_QZGE01000019.1.
DR   AlphaFoldDB; Q9HZ00; -.
DR   SMR; Q9HZ00; -.
DR   STRING; 287.DR97_4695; -.
DR   PaxDb; Q9HZ00; -.
DR   PRIDE; Q9HZ00; -.
DR   DNASU; 882403; -.
DR   EnsemblBacteria; AAG06628; AAG06628; PA3240.
DR   GeneID; 882403; -.
DR   KEGG; pae:PA3240; -.
DR   PATRIC; fig|208964.12.peg.3387; -.
DR   PseudoCAP; PA3240; -.
DR   HOGENOM; CLU_045717_5_0_6; -.
DR   InParanoid; Q9HZ00; -.
DR   OMA; PHPHRGM; -.
DR   PhylomeDB; Q9HZ00; -.
DR   BioCyc; PAER208964:G1FZ6-3299-MON; -.
DR   UniPathway; UPA00724; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008127; F:quercetin 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR012093; Pirin.
DR   InterPro; IPR008778; Pirin_C_dom.
DR   InterPro; IPR003829; Pirin_N_dom.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR13903; PTHR13903; 1.
DR   Pfam; PF02678; Pirin; 1.
DR   Pfam; PF05726; Pirin_C; 1.
DR   PIRSF; PIRSF006232; Pirin; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   3: Inferred from homology;
KW   Dioxygenase; Metal-binding; Oxidoreductase; Reference proteome.
FT   CHAIN           1..285
FT                   /note="Putative quercetin 2,3-dioxygenase PA3240"
FT                   /id="PRO_0000214071"
FT   BINDING         60
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         104
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         106
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   285 AA;  31628 MW;  A26ED494A03662D9 CRC64;
     MTRYRDVLEL HTGHPASDGA GVRLTRVIGG PSPERFDPFL MLDQFDTQNP DDYVAGFPSH
     PHRGFETVTY MLEGRMRHED HLGNRGLLKP GGVQWMTAAH GIIHSEMPEQ VEGAMRGFQL
     WVNLPAKNKL APAGYRDIEP EDVPRLETAG GVKVTVIAGR FDDGQAQQIG AVERPDTEPH
     YYDLQLPAGG RIAPRLPDGH RVLLYVYEGS LTVEGERPVE IATNRLARLS EEGELSLRSE
     AGARVLVLAG KPLHEPIVQY GPFVMNSREE IEQALRDYRD GVLAV