ID   Y324_MYCLE              Reviewed;         309 AA.
AC   Q9CCW4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Uncharacterized tRNA/rRNA methyltransferase ML0324;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=ML0324;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC29832.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL583918; CAC29832.1; ALT_INIT; Genomic_DNA.
DR   PIR; D86949; D86949.
DR   RefSeq; WP_041322375.1; NC_002677.1.
DR   AlphaFoldDB; Q9CCW4; -.
DR   SMR; Q9CCW4; -.
DR   STRING; 272631.ML0324; -.
DR   EnsemblBacteria; CAC29832; CAC29832; CAC29832.
DR   KEGG; mle:ML0324; -.
DR   Leproma; ML0324; -.
DR   eggNOG; COG0566; Bacteria.
DR   HOGENOM; CLU_021322_0_0_11; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR013123; SpoU_subst-bd.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR46429; PTHR46429; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   Pfam; PF08032; SpoU_sub_bind; 1.
DR   SMART; SM00967; SpoU_sub_bind; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00186; rRNA_methyl_3; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..309
FT                   /note="Uncharacterized tRNA/rRNA methyltransferase ML0324"
FT                   /id="PRO_0000379573"
FT   REGION          1..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         261
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         281
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         290
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   309 AA;  32796 MW;  F0A5455D39A494CF CRC64;
     MPGNSRRRGA VRKSGTNKGT TVGSGGQRRR ALEGRGPTPP AYLRPNHPAA KRNQSSPHRP
     VKRTDETETV LGRNPVLECL RAGVPATALY VALGTEVDKR LTESVMRAAD VGVAILEVPR
     TYLDRITNNH LHQGIALQVP PYHYVHSDDL LAAATDSPPA LLVALDNISD PRNLGAIVRS
     VAAFSGHGIL IPQRRSASVT AVAWRTSAGA AARIPVARAT NLTRALKVWA DQGLRVIGLD
     HDGDTALDDL DGTDPLAVVV GSEGKGLSRL VRHSCDEVVS IPMAGQVESL NASVAVGVVL
     AEIARQRRL