ID   Y327_XYLFT              Reviewed;         444 AA.
AC   Q87EI0;
DT   16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Putative zinc metalloprotease PD_0327;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=PD_0327;
OS   Xylella fastidiosa (strain Temecula1 / ATCC 700964).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xylella.
OX   NCBI_TaxID=183190;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Temecula1 / ATCC 700964;
RX   PubMed=12533478; DOI=10.1128/jb.185.3.1018-1026.2003;
RA   Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y.,
RA   Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A.,
RA   Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S.,
RA   Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M., Carrer H.,
RA   Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L.,
RA   Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L.,
RA   Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S.,
RA   Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F.,
RA   Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G.,
RA   Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A.,
RA   Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L.,
RA   Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P.;
RT   "Comparative analyses of the complete genome sequences of Pierce's disease
RT   and citrus variegated chlorosis strains of Xylella fastidiosa.";
RL   J. Bacteriol. 185:1018-1026(2003).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE009442; AAO28211.1; -; Genomic_DNA.
DR   RefSeq; WP_004089328.1; NC_004556.1.
DR   AlphaFoldDB; Q87EI0; -.
DR   SMR; Q87EI0; -.
DR   EnsemblBacteria; AAO28211; AAO28211; PD_0327.
DR   GeneID; 58015880; -.
DR   KEGG; xft:PD_0327; -.
DR   HOGENOM; CLU_025778_0_2_6; -.
DR   OMA; QYMVGFG; -.
DR   Proteomes; UP000002516; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 2.
DR   Pfam; PF17820; PDZ_6; 2.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 2.
DR   SUPFAM; SSF50156; SSF50156; 2.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Transmembrane; Transmembrane helix; Zinc.
FT   CHAIN           1..444
FT                   /note="Putative zinc metalloprotease PD_0327"
FT                   /id="PRO_0000088477"
FT   TRANSMEM        98..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          192..278
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         22
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         26
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   444 AA;  48321 MW;  31B92C0C7BB247BA CRC64;
     MGDFLASIWW MIVSFSVLVT FHEFGHYWVA RRCGVKVLRF SIGFGTPLWS RRSSSGTEFV
     IGAIPLGGYV KMLDEREADV TVAERNQAFN RKSVWQRIAI VAAGPLANLL LCMLLLWVLF
     VIGKQDYSAT VGRAEHLAAQ AGIHPGDRIT AIDGRQVTSW SEASMLLTAA AMDRQNAVLS
     VIGPYGERSE HTLELSKLKQ PFDERHVTAL VGINWQFMLQ PPIIAKIEPG SIAEGAIKPG
     DIVLAVDGQQ TLSTEDLYNQ IQKLGRDGHP GMIEIRRGEE RLALELSPRK SAQGVWLLGV
     KTNPGPVPAF DSQQRYGVLA AVPLAIRETA RMTADSLGMM KRIITGQASA KNISGPISIA
     KIANASAKRG VGWFIYFLSL LSLSLAIINL FPIPILDGGH LLYYAIELLK GSPLSTRAMA
     AGQYIGLALL AGLMGLAFYN DLLG