ID   Y3329_MYCTU             Reviewed;         466 AA.
AC   O53379; I6Y379; L0TFB0;
DT   01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-2015, sequence version 2.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Probable aminotransferase Rv3329;
DE            EC=2.6.1.-;
GN   OrderedLocusNames=Rv3329 {ECO:0000312|EMBL:CCP46150.1},
GN   RVBD_3329 {ECO:0000312|EMBL:AFN51338.1};
GN   ORFNames=P425_03469 {ECO:0000312|EMBL:KBJ27814.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genome Sequencing Platform;
RA   Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA   Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genomics Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA   Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA   Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA   Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 11-25, AND SEQUENCE REVISION TO N-TERMINUS.
RC   STRAIN=H37Rv;
RX   PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA   Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA   Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT   "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT   annotated encoding genes.";
RL   Genomics 114:292-304(2022).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=23986315; DOI=10.1128/cvi.00416-13;
RA   Jones G.J., Khatri B.L., Garcia-Pelayo M.C., Kaveh D.A., Bachy V.S.,
RA   Hogarth P.J., Wooff E., Golby P., Vordermeier H.M.;
RT   "Development of an unbiased antigen-mining approach to identify novel
RT   vaccine antigens and diagnostic reagents for bovine tuberculosis.";
RL   Clin. Vaccine Immunol. 20:1675-1682(2013).
CC   -!- FUNCTION: Probable aminotransferase. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:P53555};
CC   -!- BIOTECHNOLOGY: Was identified as a novel immunogenic protein with the
CC       potential to reduce the severity of M.bovis infection when used alone
CC       as a subunit vaccine. {ECO:0000269|PubMed:23986315}.
CC   -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFN51338.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
CC       Sequence=CCP46150.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
CC       Sequence=KBJ27814.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR   EMBL; AL123456; CCP46150.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP003248; AFN51338.1; ALT_INIT; Genomic_DNA.
DR   EMBL; JLDD01000041; KBJ27814.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_217846.3; NC_000962.3.
DR   AlphaFoldDB; O53379; -.
DR   SMR; O53379; -.
DR   STRING; 83332.Rv3329; -.
DR   PaxDb; O53379; -.
DR   PRIDE; O53379; -.
DR   DNASU; 888028; -.
DR   GeneID; 888028; -.
DR   KEGG; mtu:Rv3329; -.
DR   KEGG; mtv:RVBD_3329; -.
DR   PATRIC; fig|83332.111.peg.3714; -.
DR   TubercuList; Rv3329; -.
DR   eggNOG; COG0161; Bacteria.
DR   HOGENOM; CLU_016922_4_0_11; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0004015; F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity; IBA:GO_Central.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0009102; P:biotin biosynthetic process; IBA:GO_Central.
DR   CDD; cd00610; OAT_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR005814; Aminotrans_3.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00202; Aminotran_3; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
PE   1: Evidence at protein level;
KW   Aminotransferase; Direct protein sequencing; Pyridoxal phosphate;
KW   Reference proteome; Transferase.
FT   CHAIN           1..466
FT                   /note="Probable aminotransferase Rv3329"
FT                   /id="PRO_0000432513"
FT   MOD_RES         294
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250|UniProtKB:P53555"
SQ   SEQUENCE   466 AA;  50843 MW;  F129E9529D973E3A CRC64;
     MPRIRKPPSR SLPEPSAALA NTTTRNLWLH FARHGAGIQH PVIVRGDGVT IFDDRGKSYL
     DALSGLFVVQ VGYGRAELAE AAARQAGTLG YFPLWGYATP PAIELAERLA RYAPGDLNRV
     FFTSGGTEAV ETAWKVAKQY FKLTGKPGKQ KVISRSIAYH GTTQGALAIT GLPLFKAPFE
     PLTPGGFRVP NTNFYRAPLH TDLKEFGRWA ADRIAEAIEF EGPDTVAAVF LEPVQNAGGC
     IPAPPGYFER VREICDRYDV LLVSDEVICA FGRIGSMFAC EDLGYVPDMI TCAKGLTSGY
     SPLGAMIASD RLFEPFNDGE TMFAHGYTFG GHPVSAAVGL ANLDIFEREG LSDHVKRNSP
     ALRATLEKLY DLPIVGDIRG EGYFFGIELV KDQATKQTFT DDERARLLGQ VSAALFEAGL
     YCRTDDRGDP VVQVAPPLIS GQPEFDTIET ILRSVLTDTG RKYLHL