Y3330_ARATH
ID Y3330_ARATH Reviewed; 743 AA.
AC Q0WVZ1; Q680B0; Q8L8U3; Q9M9P8;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Putative metallophosphoesterase At3g03305;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN OrderedLocusNames=At3g03305; ORFNames=T17B22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC Note=Divalent metal cation. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26098.1; Type=Erroneous gene model prediction; Note=The predicted gene At3g03300 has been split into 2 genes: At3g03300 and At3g03305.; Evidence={ECO:0000305};
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DR EMBL; AC012328; AAF26098.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73927.1; -; Genomic_DNA.
DR EMBL; AK175957; BAD43720.1; -; mRNA.
DR EMBL; AK226592; BAE98707.1; -; mRNA.
DR EMBL; AY088809; AAM67119.1; -; mRNA.
DR RefSeq; NP_566200.1; NM_111201.4.
DR AlphaFoldDB; Q0WVZ1; -.
DR BioGRID; 6624; 3.
DR IntAct; Q0WVZ1; 1.
DR STRING; 3702.AT3G03305.1; -.
DR PaxDb; Q0WVZ1; -.
DR PRIDE; Q0WVZ1; -.
DR ProteomicsDB; 234658; -.
DR EnsemblPlants; AT3G03305.1; AT3G03305.1; AT3G03305.
DR GeneID; 821291; -.
DR Gramene; AT3G03305.1; AT3G03305.1; AT3G03305.
DR KEGG; ath:AT3G03305; -.
DR Araport; AT3G03305; -.
DR TAIR; locus:505006328; AT3G03305.
DR eggNOG; KOG0701; Eukaryota.
DR HOGENOM; CLU_018027_0_0_1; -.
DR InParanoid; Q0WVZ1; -.
DR OMA; VEWQTYH; -.
DR OrthoDB; 986462at2759; -.
DR PhylomeDB; Q0WVZ1; -.
DR PRO; PR:Q0WVZ1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q0WVZ1; baseline and differential.
DR Genevisible; Q0WVZ1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF00149; Metallophos; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Membrane; Metal-binding; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..40
FT /evidence="ECO:0000255"
FT CHAIN 41..743
FT /note="Putative metallophosphoesterase At3g03305"
FT /id="PRO_0000404659"
FT TRANSMEM 514..534
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 565..585
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 623..643
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 687..704
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 716..736
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 66
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 68
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 101
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT CONFLICT 303
FT /note="N -> K (in Ref. 3; BAD43720)"
FT /evidence="ECO:0000305"
FT CONFLICT 498..501
FT /note="NEFR -> SEFL (in Ref. 4; AAM67119)"
FT /evidence="ECO:0000305"
FT CONFLICT 522
FT /note="L -> F (in Ref. 4; AAM67119)"
FT /evidence="ECO:0000305"
FT CONFLICT 591
FT /note="A -> T (in Ref. 4; AAM67119)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 743 AA; 84747 MW; 4FC442A712343DAE CRC64;
MESIGDDDEL RSKTVSLPRR ISFTILLLLL LISLSTRVSG SLDKSGRRVI EARTGQDLIW
VVQLSDLHFS VHHPERAIDF KNIVGPALAL INPSLVLITG DLTDGKSKDM LTMKLNEDEW
LEYESVMQDV VKRSGLNKSI FYDLRGNHDN FGVPSVGSSV DFFSKYSING QMGRKGNVNT
ITVETSERKH LFVGIDTTMH IGLRGPTNLF GHPTDELLSS LDSHLSQWDN QSAKPVAKIS
FGHFPLSFTA LSHSQKSLKD VFLKHSISAY LCGHLHSRFG KNLKRHHHSG GISLSDNDLF
QLNMRQSGAE STSNCSFGAL PAAEFWEWEM GDWRKNRAMR IMAIDRGHVS YVDLDFKSKP
QKTIILPTFP LDSRFMSTSF ARHKYECQHM ISSSYDAIRA IVFSHSLVVE VVARVYDSSP
GFDNLVMEAP MRKHGGDSSS SGATFFSLPW NYRAFEDPLP DRFWLQIEVT DIKGRLTLSE
MRPFSINGLS SKVSWTWNEF RVMGCQWAAL YYPILWPALY SLFLVFLIPK CIIIVFKKQY
TLKKFIAKKG PITLVLWILQ DLCRMPVVWF GYMAYLFYLI FFPWFSGEVF ADSGDRAYMT
IMGWVVTSSG ADRKHEYIGQ PDVMVVVIPH VVFVVIPSVL VVCCLVAERE IYKDHIRTVS
GKKEDDHDRG RKKRSQRRSL LFSNRRLFRK SVLLASLALY WKHFKNCWAL GRAYEMNVVH
FPGYSLVVPL LLLYVICKTH KVP
