ID   Y3371_MYCTO             Reviewed;         446 AA.
AC   P9WKA8; L0TDY3; O50400;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT3481;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT3481;
GN   OrderedLocusNames=MT3481;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK47818.1; -; Genomic_DNA.
DR   PIR; B70972; B70972.
DR   RefSeq; WP_010924648.1; NC_002755.2.
DR   AlphaFoldDB; P9WKA8; -.
DR   SMR; P9WKA8; -.
DR   EnsemblBacteria; AAK47818; AAK47818; MT3481.
DR   KEGG; mtc:MT3481; -.
DR   HOGENOM; CLU_024186_3_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..446
FT                   /note="Putative diacyglycerol O-acyltransferase MT3481"
FT                   /id="PRO_0000427686"
FT   REGION          425..446
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        129
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   446 AA;  48794 MW;  AF5FC07E8FA1A375 CRC64;
     MAQLTALDAG FLKSRDPERH PGLAIGAVAV VNGAAPSYDQ LKTVLTERIK SIPRCTQVLA
     TEWIDYPGFD LTQHVRRVAL PRPGDEAELF RAIALALERP LDPDRPLWEC WIIEGLNGNR
     WAILIKIHHC MAGAMSAAHL LARLCDDADG SAFANNVDIK QIPPYGDARS WAETLWRMSV
     SIAGAVCTAA ARAVSWPAVT SPAGPVTTRR RYQAVRVPRD AVDAVCHKFG VTANDVALAA
     ITEGFRTVLL HRGQQPRADS LRTLEKTDGS SAMLPYLPVE YDDPVRRLRT VHNRSQQSGR
     RQPDSLSDYT PLMLCAKMIH ALARLPQQGI VTLATSAPRP RHQLRLMGQK MDQVLPIPPT
     ALQLSTGIAV LSYGDELVFG ITADYDAASE MQQLVNGIEL GMARLVALSD DSVLLFTKDR
     RKASSRALPS AARRGRPSVP TARARH