ID   Y3381_CLOBM             Reviewed;         645 AA.
AC   B1KSX3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=UPF0313 protein CLK_3381 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=CLK_3381;
OS   Clostridium botulinum (strain Loch Maree / Type A3).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=498214;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Loch Maree / Type A3;
RX   PubMed=18060065; DOI=10.1371/journal.pone.0001271;
RA   Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C.,
RA   Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.;
RT   "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4
RT   and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within
RT   plasmids.";
RL   PLoS ONE 2:E1271-E1271(2007).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; CP000962; ACA55602.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1KSX3; -.
DR   EnsemblBacteria; ACA55602; ACA55602; CLK_3381.
DR   KEGG; cbl:CLK_3381; -.
DR   HOGENOM; CLU_018288_2_0_9; -.
DR   OMA; YKLEYYE; -.
DR   Proteomes; UP000000722; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..645
FT                   /note="UPF0313 protein CLK_3381"
FT                   /id="PRO_1000139929"
FT   DOMAIN          295..566
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          598..645
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         309
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         313
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         316
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   645 AA;  74517 MW;  8E5CBA224D83971C CRC64;
     MSNMDFLPIS KEDLKKRNID VLDFIVVTGD AYVDHPSFGT AIIGRVLERE GFTVGIIAQP
     NWNNIEDFKK LGKPKYGFLV NSGNIDSMVN HYTASKKKRH DDFYSPGGKS GYRPDRAVIV
     YCNKIKEAFK DSPIIIGGIE ASLRRFAHYD YWDNSVRRSI LEDSSADLLI YGMGEKPIVQ
     VSNLLRYGMK IDSIKNVRGT TYIEKDISSL KDYIEIPSFE EVSTNKKSYA EAYKIQYYEQ
     DSIRGKTLVQ KHKERYVVQN PPQPPLSQEE MDEVYALPYA RTYHPMYEAE GGIPAIKEVK
     FSITSHRGCY GSCSFCALTF HQGRVIQNRS QDSILKEANM MTNMKDFKGY IHDVGGPTAN
     FRHRACKVQE KHGTCKNKQC VFPKACKNLI VDHKEYLSLL RKIRKIPNVK KVFIRSGIRF
     DYLMYDKNDE FFKELCEHHI SGQLKVAPEH ISDKVLNLMG KPTRNVYDSF VKKYYDINKK
     IHKNQFLVPY LMSSHPGSDL KAAIELAQYI KKMGYTPEQV QDFYPTPGSL STTMYYTGIN
     PLTEEKVYVP KDQKEKRMQR ALLQFSILDN YDLVKEALIK AHREDLIGNG PDCLIPYNKP
     YKKSHKKNNV KNNNNHYNKN NNYNKNKDVS KKNKKNSLSK HKKRK