ID   Y338_RICFE              Reviewed;         412 AA.
AC   Q4UML9;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Uncharacterized zinc protease RF_0338;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=RF_0338;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Divalent metal cations. Binds Zn(2+). {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000053; AAY61189.1; -; Genomic_DNA.
DR   RefSeq; WP_011270681.1; NC_007109.1.
DR   AlphaFoldDB; Q4UML9; -.
DR   SMR; Q4UML9; -.
DR   STRING; 315456.RF_0338; -.
DR   MEROPS; M16.016; -.
DR   EnsemblBacteria; AAY61189; AAY61189; RF_0338.
DR   KEGG; rfe:RF_0338; -.
DR   eggNOG; COG0612; Bacteria.
DR   HOGENOM; CLU_009902_3_0_5; -.
DR   OMA; WSNPDNV; -.
DR   OrthoDB; 1188251at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; SSF63411; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Zinc.
FT   CHAIN           1..412
FT                   /note="Uncharacterized zinc protease RF_0338"
FT                   /id="PRO_0000295170"
FT   ACT_SITE        52
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         49
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         53
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         129
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
SQ   SEQUENCE   412 AA;  46447 MW;  397AC841E59F440A CRC64;
     MKENFNISKL KNGLTVLTYN MPYVDSVAIN LITKVGSRYE NSEEEGISHF LEHMAFKGTT
     TRTAKQIAEE FDEIGGHFNA YTGHEKTIYY ARVLSENCDK ALNILADIIQ NSIFAEEEIA
     KEYQVILQEI AHSQDNPDDL IYEKFYSSVY KDQPLGKPIL GASKTLSSFT KEHFLSFIDK
     HYNAGNLYLS VAGNVDHDKI VSSAERLFSS LKQGEKSNFL PAKYIGGNSF INKDLEQTTL
     ILGFEGTPYI NLERLYRTQL LAIIFGGGMS SRLFQHIREK LGLAYAVGSY NSTYSDSGVF
     TIYASTAHDK LELLYKELKT EITKMTEKVN EEEMIRAKTQ LRSNLLMAQE KVAYKSEEIG
     KNYAAFGKYI PPEEIMEIIT NIKADDIINT ANKIFSSITT SAIIGPNDLR GF