ID   Y343_BUCAP              Reviewed;         264 AA.
AC   Q8K9J1;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Uncharacterized metal-dependent hydrolase BUsg_343 {ECO:0000305};
DE            EC=3.1.-.- {ECO:0000305};
GN   OrderedLocusNames=BUsg_343;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250|UniProtKB:P0AFQ7};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000250|UniProtKB:P0AFQ7};
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       TatD-type hydrolase family. {ECO:0000305}.
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DR   EMBL; AE013218; AAM67897.1; -; Genomic_DNA.
DR   RefSeq; WP_011053864.1; NC_004061.1.
DR   AlphaFoldDB; Q8K9J1; -.
DR   SMR; Q8K9J1; -.
DR   STRING; 198804.BUsg_343; -.
DR   PRIDE; Q8K9J1; -.
DR   EnsemblBacteria; AAM67897; AAM67897; BUsg_343.
DR   KEGG; bas:BUsg_343; -.
DR   eggNOG; COG0084; Bacteria.
DR   HOGENOM; CLU_031506_4_0_6; -.
DR   OMA; PNEAPRI; -.
DR   OrthoDB; 915852at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0004536; F:deoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01310; TatD_DNAse; 1.
DR   InterPro; IPR018228; DNase_TatD-rel_CS.
DR   InterPro; IPR015991; Hydrolase_TatD-type.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR001130; TatD-like.
DR   Pfam; PF01026; TatD_DNase; 1.
DR   PIRSF; PIRSF005902; DNase_TatD; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR00010; TIGR00010; 1.
DR   PROSITE; PS01137; TATD_1; 1.
DR   PROSITE; PS01091; TATD_3; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding.
FT   CHAIN           1..264
FT                   /note="Uncharacterized metal-dependent hydrolase BUsg_343"
FT                   /id="PRO_0000202003"
FT   BINDING         7
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         9
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         100
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         100
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         136
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         161
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         211
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
SQ   SEQUENCE   264 AA;  30703 MW;  0243E021286E5FC5 CRC64;
     MFLIDSHCHI DQMNIDQKND DLLHQEIEKI LNKAYENHVK KFLTVSTSIQ NFYKTKKLLS
     TYDSIFYSCG IHPLYCQKEI ECLNELKKLS YDKCVIALGE TGLDYYYSFE TKNLQEKFFR
     EHIRIAITLN KPIIIHSRNA IKDTIKILKE ENAEKCGGIL HSFTESEKSA FELLDMGFYI
     SFSGIITFKK SIELCNTLKK IPLEKLLIET DSPYLAPIPY RGKKNQPAYL LDIAKKIALI
     KEISLKELAK ITTNNFFTLF NLNF