ID   Y3480_MYCTO             Reviewed;         497 AA.
AC   P9WKA6; L0TE90; O06343;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT3584;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT3584;
GN   OrderedLocusNames=MT3584;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47943.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK47943.1; ALT_INIT; Genomic_DNA.
DR   PIR; E70568; E70568.
DR   RefSeq; WP_023641844.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKA6; -.
DR   SMR; P9WKA6; -.
DR   EnsemblBacteria; AAK47943; AAK47943; MT3584.
DR   KEGG; mtc:MT3584; -.
DR   PATRIC; fig|83331.31.peg.3859; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..497
FT                   /note="Putative diacyglycerol O-acyltransferase MT3584"
FT                   /id="PRO_0000427687"
FT   ACT_SITE        143
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   497 AA;  53301 MW;  12CA8F112B564D37 CRC64;
     MSQTARRLGP QDMFFLYSES STTMMHVGAL MPFTPPSGAP PDLLRQLVDE SKASEVVEPW
     SLRLSHPELL YHPTQSWVVD DNFDLDYHVR RSALASPGDE RELGIPVSRL HSHALDLRRP
     PWEVHFIEGL EGGRFAIYIK MHHSLIDGYT GQKMLARSLS TDPHDTTHPL FFNIPTPGRS
     PADTQDSVGG GLIAGAGNVL DGLGDVVRGL GGLVSGVGSV LGSVAGAGRS TFELTKALVN
     AQLRSDHEYR NLVGSVQAPH CILNTRISRN RRFATQQYPL DRLKAIGAQY DATINDVALA
     IIGGGLRRFL DELGELPNKS LIVVLPVNVR PKDDEGGGNA VATILATLGT DVADPVQRLA
     AVTASTRAAK AQLRSMDKDA ILAYSAALMA PYGVQLASTL SGVKPPWPYT FNLCVSNVPG
     PEDVLYVRGS RMEASYPVSL VAHSQALNVT LQSYAGTLNF GFIGCRDTLP HLQRLAVYTG
     EALDQLAAAD GAAGLGS