Y3514_ARATH
ID Y3514_ARATH Reviewed; 337 AA.
AC Q8W566; Q9LIM1;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Uncharacterized exonuclease domain-containing protein At3g15140;
DE EC=3.1.-.-;
GN OrderedLocusNames=At3g15140; ORFNames=F4B12.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02568.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At3g15130 and At3g15140.; Evidence={ECO:0000305};
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DR EMBL; AP001299; BAB02568.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75624.1; -; Genomic_DNA.
DR EMBL; AF419612; AAL31944.1; -; mRNA.
DR EMBL; AY079112; AAL84996.1; -; mRNA.
DR RefSeq; NP_566502.1; NM_112377.2.
DR AlphaFoldDB; Q8W566; -.
DR SMR; Q8W566; -.
DR STRING; 3702.AT3G15140.1; -.
DR PaxDb; Q8W566; -.
DR PRIDE; Q8W566; -.
DR ProteomicsDB; 234635; -.
DR EnsemblPlants; AT3G15140.1; AT3G15140.1; AT3G15140.
DR GeneID; 820745; -.
DR Gramene; AT3G15140.1; AT3G15140.1; AT3G15140.
DR KEGG; ath:AT3G15140; -.
DR Araport; AT3G15140; -.
DR TAIR; locus:2083636; AT3G15140.
DR eggNOG; KOG0542; Eukaryota.
DR HOGENOM; CLU_037266_2_0_1; -.
DR InParanoid; Q8W566; -.
DR OrthoDB; 809000at2759; -.
DR PhylomeDB; Q8W566; -.
DR PRO; PR:Q8W566; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8W566; baseline and differential.
DR Genevisible; Q8W566; AT.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0016891; F:endoribonuclease activity, producing 5'-phosphomonoesters; IMP:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IMP:TAIR.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 2: Evidence at transcript level;
KW Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome.
FT CHAIN 1..337
FT /note="Uncharacterized exonuclease domain-containing
FT protein At3g15140"
FT /id="PRO_0000355993"
FT DOMAIN 129..304
FT /note="Exonuclease"
FT REGION 42..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 135
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT ACT_SITE 291
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT BINDING 133
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 133
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 234
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 291
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250"
FT BINDING 296
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
SQ SEQUENCE 337 AA; 38670 MW; EC02694DE6377E72 CRC64;
MASAFSAFRV SLSRISPFRD TRFSYPATLA LAHTKRIMCN SSHSVSPSPS PSDFSSSSSS
SSSSPSTFSL METSENARWR PMCLYYTHGK CTKMDDPAHL EIFNHDCSKE LRVAAADLER
KKSQEFNFFL VIDLEGKVEI LEFPILIVDA KTMEVVDLFH RFVRPTKMSE QAINKYIEGK
YGELGVDRVW HDTAIPFKQV VEEFEVWLAE HDLWDKDTDW GLNDAAFVTC GNWDIKTKIP
EQCVVSNINL PPYFMEWINL KDVYLNFYGR EARGMVSMMR QCGIKLMGSH HLGIDDTKNI
TRVVQRMLSE GAVLKLTARR SKSNMRNVEF LFKNRIK
