ID   Y355_BUCAI              Reviewed;         264 AA.
AC   P57436;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Uncharacterized metal-dependent hydrolase BU355 {ECO:0000305};
DE            EC=3.1.-.- {ECO:0000305};
GN   OrderedLocusNames=BU355;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250|UniProtKB:P0AFQ7};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000250|UniProtKB:P0AFQ7};
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       TatD-type hydrolase family. {ECO:0000305}.
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DR   EMBL; BA000003; BAB13059.1; -; Genomic_DNA.
DR   RefSeq; NP_240173.1; NC_002528.1.
DR   RefSeq; WP_009874311.1; NC_002528.1.
DR   AlphaFoldDB; P57436; -.
DR   SMR; P57436; -.
DR   STRING; 107806.10039025; -.
DR   PRIDE; P57436; -.
DR   EnsemblBacteria; BAB13059; BAB13059; BAB13059.
DR   KEGG; buc:BU355; -.
DR   PATRIC; fig|107806.10.peg.368; -.
DR   eggNOG; COG0084; Bacteria.
DR   HOGENOM; CLU_031506_4_0_6; -.
DR   OMA; PNEAPRI; -.
DR   BioCyc; BAPH107806:GBZJ-348-MON; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0004536; F:deoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01310; TatD_DNAse; 1.
DR   InterPro; IPR018228; DNase_TatD-rel_CS.
DR   InterPro; IPR015991; Hydrolase_TatD-type.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR001130; TatD-like.
DR   Pfam; PF01026; TatD_DNase; 1.
DR   PIRSF; PIRSF005902; DNase_TatD; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR00010; TIGR00010; 1.
DR   PROSITE; PS01137; TATD_1; 1.
DR   PROSITE; PS01090; TATD_2; 1.
DR   PROSITE; PS01091; TATD_3; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Reference proteome.
FT   CHAIN           1..264
FT                   /note="Uncharacterized metal-dependent hydrolase BU355"
FT                   /id="PRO_0000202002"
FT   BINDING         7
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         9
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         102
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         102
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         138
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         163
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
FT   BINDING         213
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFQ7"
SQ   SEQUENCE   264 AA;  30520 MW;  7F1DA900018E0AAC CRC64;
     MFLIDSHCHL DRLNYNLPLE NIEDVLKKSY QNHVKNFLTV STCISNFYNI KKLFKKYNTI
     FYSCGVHPLN CKKELNLFHT IENLSNEIKK LSCIKDVIAL GETGLDYYYS SDTKKIQQDF
     FREHIRVAIK LKKPIIVHSR NASEDTIKIL QEENAEKCKG VLHSFTGDYN TACKLLDLGF
     YISCSGIITF KNSLELCKTI RKIPLNRLLI ETDSPYLSPA PYRGKGNQPA YLFYIAEYLS
     ILKEIDIHAL GHITTSNFRT LFNI