ID   Y355_PYRFU              Reviewed;         149 AA.
AC   Q8U3V0;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=VapC ribonuclease PF0355;
DE            Short=RNase PF0355;
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Putative toxin PF0355;
GN   OrderedLocusNames=PF0355;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 2-149.
RG   Southeast collaboratory for structural genomics (SECSG);
RA   Horanyi P., Tempel W., Habel J., Chen L., Lee D., Nguyen D., Chang S.-H.,
RA   Florence Q., Zhou W., Lin D., Zhang H., Praissman J., Jenney F.E. Jr.,
RA   Adams M.W.W., Liu Z.-J., Rose J.P., Wang B.C.;
RT   "Conserved hypothetical protein Pfu-367848-001 from Pyrococcus furiosus.";
RL   Submitted (DEC-2004) to the PDB data bank.
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE009950; AAL80479.1; -; Genomic_DNA.
DR   RefSeq; WP_014835128.1; NZ_CP023154.1.
DR   PDB; 1Y82; X-ray; 2.30 A; A/B/C/D=2-149.
DR   PDBsum; 1Y82; -.
DR   AlphaFoldDB; Q8U3V0; -.
DR   SMR; Q8U3V0; -.
DR   PRIDE; Q8U3V0; -.
DR   EnsemblBacteria; AAL80479; AAL80479; PF0355.
DR   GeneID; 41712180; -.
DR   KEGG; pfu:PF0355; -.
DR   PATRIC; fig|186497.12.peg.368; -.
DR   eggNOG; arCOG02224; Archaea.
DR   HOGENOM; CLU_142042_0_0_2; -.
DR   OMA; LMISVEM; -.
DR   OrthoDB; 98964at2157; -.
DR   PhylomeDB; Q8U3V0; -.
DR   EvolutionaryTrace; Q8U3V0; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   InterPro; IPR016647; VapV_Thermo.
DR   Pfam; PF01850; PIN; 1.
DR   PIRSF; PIRSF016154; NA-bd_PIN_PH0500; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..149
FT                   /note="VapC ribonuclease PF0355"
FT                   /id="PRO_0000407905"
FT   DOMAIN          8..122
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         10
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         98
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   STRAND          6..9
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           11..17
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           20..22
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           23..32
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   STRAND          33..38
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           39..51
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           56..66
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   STRAND          67..70
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           74..88
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   TURN            89..91
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           96..108
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   STRAND          111..115
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           117..126
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   STRAND          130..132
FT                   /evidence="ECO:0007829|PDB:1Y82"
FT   HELIX           133..147
FT                   /evidence="ECO:0007829|PDB:1Y82"
SQ   SEQUENCE   149 AA;  17153 MW;  9D208710672BBD85 CRC64;
     MPLPPDITFD SLALIKMHSQ SMKKILEITL AKFTVNLSIV TVYRYLTVRA YLKKNIELEL
     DVLKDIYNIV PLNEEIAIKA AQIEADLMRK GMMPDIEDVL TAATAIYTKS LLITDDSKRY
     EPMRRFGLDT MPLDKFVKEV ELMVEKELI