ID   CARD_MYCS2              Reviewed;         162 AA.
AC   A0R561;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=RNA polymerase-binding transcription factor CarD;
GN   Name=carD; OrderedLocusNames=MSMEG_6077, MSMEI_5917;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, INTERACTION WITH RPOB, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=19596241; DOI=10.1016/j.cell.2009.04.041;
RA   Stallings C.L., Stephanou N.C., Chu L., Hochschild A., Nickels B.E.,
RA   Glickman M.S.;
RT   "CarD is an essential regulator of rRNA transcription required for
RT   Mycobacterium tuberculosis persistence.";
RL   Cell 138:146-159(2009).
RN   [5]
RP   INTERACTION WITH RPOB, AND MUTAGENESIS OF ARG-25 AND ARG-47.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22904282; DOI=10.1128/jb.00879-12;
RA   Weiss L.A., Harrison P.G., Nickels B.E., Glickman M.S., Campbell E.A.,
RA   Darst S.A., Stallings C.L.;
RT   "Interaction of CarD with RNA polymerase mediates Mycobacterium
RT   tuberculosis viability, rifampicin resistance, and pathogenesis.";
RL   J. Bacteriol. 194:5621-5631(2012).
CC   -!- FUNCTION: Controls rRNA transcription by binding to the RNA polymerase
CC       (RNAP). Required for the stringent response to starvation, oxidative
CC       and genotoxic stresses as well as control of rRNA under normal growth
CC       conditions. {ECO:0000269|PubMed:19596241}.
CC   -!- SUBUNIT: Interacts directly with the N-terminus of the RNA polymerase
CC       subunit beta (RpoB). {ECO:0000269|PubMed:19596241,
CC       ECO:0000269|PubMed:22904282}.
CC   -!- INTERACTION:
CC       A0R561; P60281: rpoB; NbExp=2; IntAct=EBI-2408339, EBI-2408357;
CC   -!- INDUCTION: Induced by DNA damage and starvation.
CC       {ECO:0000269|PubMed:19596241}.
CC   -!- DISRUPTION PHENOTYPE: Essential for growth, it cannot be disrupted.
CC       {ECO:0000269|PubMed:19596241}.
CC   -!- SIMILARITY: Belongs to the CarD family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK72642.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42350.1; -; Genomic_DNA.
DR   RefSeq; WP_011731020.1; NZ_SIJM01000046.1.
DR   RefSeq; YP_890299.1; NC_008596.1.
DR   AlphaFoldDB; A0R561; -.
DR   SMR; A0R561; -.
DR   IntAct; A0R561; 4.
DR   STRING; 246196.MSMEI_5917; -.
DR   EnsemblBacteria; ABK72642; ABK72642; MSMEG_6077.
DR   EnsemblBacteria; AFP42350; AFP42350; MSMEI_5917.
DR   GeneID; 66737361; -.
DR   KEGG; msg:MSMEI_5917; -.
DR   KEGG; msm:MSMEG_6077; -.
DR   PATRIC; fig|246196.19.peg.5915; -.
DR   eggNOG; COG1329; Bacteria.
DR   OMA; DMKVMIP; -.
DR   OrthoDB; 1942592at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   Gene3D; 1.20.58.1290; -; 1.
DR   InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR   InterPro; IPR003711; CarD-like/TRCF_domain.
DR   InterPro; IPR042215; CarD-like_C.
DR   Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR   SMART; SM01058; CarD_TRCF; 1.
DR   SUPFAM; SSF141259; SSF141259; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..162
FT                   /note="RNA polymerase-binding transcription factor CarD"
FT                   /id="PRO_0000420261"
FT   MUTAGEN         25
FT                   /note="R->E: Weakens interaction with RpoB, decreases
FT                   growth rate, and increases rifampicin and streptomycin
FT                   sensitivity."
FT                   /evidence="ECO:0000269|PubMed:22904282"
FT   MUTAGEN         47
FT                   /note="R->E: Weakens interaction with RpoB, increases
FT                   rifampicin sensitivity, but does not affect growth rate."
FT                   /evidence="ECO:0000269|PubMed:22904282"
SQ   SEQUENCE   162 AA;  17934 MW;  DFC6B1D66F5C1A78 CRC64;
     MIFKVGDTVV YPHHGAALIE AIETRTIKGE QKEYLVLKVA QGDLTVRVPA DNAEYVGVRD
     VVGQEGLDKV FQVLRAPHTE EPTNWSRRYK ANLEKLASGD VNKVAEVVRD LWRRDQERGL
     SAGEKRMLAK ARQILVGELA LAENTDDAKA ETILDEVLAA AS