ID   CARD_NEUCR              Reviewed;         533 AA.
AC   Q1K615; V5IL17;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Beta-apo-4'-carotenal oxygenase;
DE            EC=1.2.1.82;
DE   AltName: Full=Beta-apo-4'-carotenal dehydrogenase;
DE   AltName: Full=Yellow protein 1;
GN   Name=ylo-1; ORFNames=NCU04013;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [2]
RP   FUNCTION.
RX   PubMed=18812228; DOI=10.1016/j.fgb.2008.09.001;
RA   Estrada A.F., Maier D., Scherzinger D., Avalos J., Al-Babili S.;
RT   "Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new
RT   biosynthetic reaction sequence leading to neurosporaxanthin formation.";
RL   Fungal Genet. Biol. 45:1497-1505(2008).
RN   [3]
RP   CATALYTIC ACTIVITY, FUNCTION, AND INDUCTION.
RX   PubMed=18627463; DOI=10.1111/j.1365-2958.2008.06349.x;
RA   Estrada A.F., Youssar L., Scherzinger D., Al-Babili S., Avalos J.;
RT   "The ylo-1 gene encodes an aldehyde dehydrogenase responsible for the last
RT   reaction in the Neurospora carotenoid pathway.";
RL   Mol. Microbiol. 69:1207-1220(2008).
RN   [4]
RP   FUNCTION.
RX   PubMed=19007949; DOI=10.1016/j.phytochem.2008.09.016;
RA   Sandmann G., Takaichi S., Fraser P.D.;
RT   "C(35)-apocarotenoids in the yellow mutant Neurospora crassa YLO.";
RL   Phytochemistry 69:2886-2890(2008).
CC   -!- FUNCTION: Beta-apo-4'-carotenal oxygenase involved in the last step of
CC       synthesis of neurosporaxanthin, a carboxylic apocarotenoid acting as an
CC       essential protective pigment and leading to orange pigmentation.
CC       {ECO:0000269|PubMed:18627463, ECO:0000269|PubMed:18812228,
CC       ECO:0000269|PubMed:19007949}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4'-apo-beta-carotenal + H2O + NAD(+) = 2 H(+) + NADH +
CC         neurosporaxanthin; Xref=Rhea:RHEA:31515, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:53157, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:63069; EC=1.2.1.82;
CC         Evidence={ECO:0000269|PubMed:18627463};
CC   -!- INDUCTION: Is not regulated by light unlike the genes responsible for
CC       the preceding reactions in the carotenoid pathway.
CC       {ECO:0000269|PubMed:18627463}.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; CM002241; ESA42262.1; -; Genomic_DNA.
DR   RefSeq; XP_011394899.1; XM_011396597.1.
DR   AlphaFoldDB; Q1K615; -.
DR   SMR; Q1K615; -.
DR   STRING; 5141.EFNCRP00000003551; -.
DR   EnsemblFungi; ESA42262; ESA42262; NCU04013.
DR   GeneID; 3873815; -.
DR   KEGG; ncr:NCU04013; -.
DR   VEuPathDB; FungiDB:NCU04013; -.
DR   HOGENOM; CLU_005391_3_1_1; -.
DR   InParanoid; Q1K615; -.
DR   OMA; RHGKRWM; -.
DR   BRENDA; 1.2.1.82; 3627.
DR   Proteomes; UP000001805; Chromosome 5, Linkage Group VI.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IBA:GO_Central.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   InterPro; IPR012394; Aldehyde_DH_NAD(P).
DR   PANTHER; PTHR43570; PTHR43570; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PIRSF; PIRSF036492; ALDH; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..533
FT                   /note="Beta-apo-4'-carotenal oxygenase"
FT                   /id="PRO_0000418442"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        260
FT                   /evidence="ECO:0000250"
FT   BINDING         204..209
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   533 AA;  59504 MW;  E90227192B578FB9 CRC64;
     MAASKVEIAP FEVTPLDAIP AVCSTARATF ASHKTKNLQW RLVQLRKLYW ALDDFKASLM
     AALQQDLRKG GYESDFTEVD WVKNDCLHMI NNLETFAKTE KLKDLPVTYS MMNFRVKKEP
     LGTVLIIGPY NFPIQLVLAP LVGAIGAGCT AVIKPSELTP ACAMAMKEMI ESRLDRDAFA
     VVNGGVPETN ALMEEKWDKI MFTGSAQVGS IIARKAAETL TPVCLELGGR NPAFVTKKAN
     LALAARRLMW GKVLNAGQVC MSHNYVLVDK DVADTFIEFL KIAYKDMFPN GAKASPDLSR
     IVNARHFNRI KKMLDETKGK IVMGGEMDES ELYIEPTAVL VDSLDDPMMQ EESFGPIFSI
     YPVDTLDQAL SIANNVHRTP LALMAFGDKS ETNRILDEMT SGGACINDSY FHGAVHTVPF
     GGVGDSGWGA YRGKASFDNF THFRTVSETP TWMDRFLRVR YMPYDWSELR LLQRWTNKKP
     NFDRQGTVAK GSEYWMWYFL GLGTKGGVKG ALMRWLVVVA GYYLSAYMKA RRA