ID   CARE_STRTH              Reviewed;         388 AA.
AC   P21542;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=4''-mycarosyl isovaleryl-CoA transferase;
DE            EC=2.-.-.-;
GN   Name=carE; Synonyms=acyB1;
OS   Streptomyces thermotolerans.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=80858;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11416 / CBS 960.69 / DSM 40277 / JCM 4519 / NBRC 13088 / NRRL
RC   B-2345 / VKM Ac-1821;
RX   PubMed=2628170; DOI=10.1016/0378-1119(89)90421-6;
RA   Epp J.K., Huber M.L.B., Turner J.R., Goodson T., Schoner B.E.;
RT   "Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens
RT   and Streptomyces lividans by introduction of a cloned carbomycin
RT   biosynthetic gene from Streptomyces thermotolerans.";
RL   Gene 85:293-301(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7764361; DOI=10.1271/bbb.57.2020;
RA   Arisawa A., Kawamura N., Tsunekawa H., Okamura K., Tone H., Okamoto R.;
RT   "Cloning and nucleotide sequences of two genes involved in the 4''-O-
RT   acylation of macrolide antibiotics from Streptomyces thermotolerans.";
RL   Biosci. Biotechnol. Biochem. 57:2020-2025(1993).
CC   -!- FUNCTION: Catalyzes the acylation of the mycaminose sugar during
CC       carbomycin biosynthesis. Recombinant strains containing care can use
CC       spiramycin as a substrate and convert it to the hybrid antibiotic,
CC       isovaleryl spiramycin.
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DR   EMBL; D31821; BAA06607.1; -; Genomic_DNA.
DR   AlphaFoldDB; P21542; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002656; Acyl_transf_3_dom.
DR   Pfam; PF01757; Acyl_transf_3; 1.
PE   4: Predicted;
KW   Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..388
FT                   /note="4''-mycarosyl isovaleryl-CoA transferase"
FT                   /id="PRO_0000089319"
FT   REGION          366..388
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   388 AA;  43561 MW;  88E322E488CBF5EC CRC64;
     MPLPKHLPAL GGMRFISALL VFTSHISTQP FFKNTEINSA LQFPLNRLGP LTVSFFFMLS
     GFVLTWAGLP DKSKVNFWRR RTVRAYSLHL PVLLVTLLIV LALNEPNMGR SVWDGLLTNL
     LLIQAWFPDH HEYGSMNPVA WSLSCELFFY AMFPFLFAFF TKVRTDRLWR WAAAVSVAAV
     SIPLVALLLP ASPPLPWDPD MPQLRWWFIY MFPPVRLLEF VLGMLMAQIV IRGRWRGPRP
     LACVALFSAV FAVTFAVPNH YDPGALTVPV IALLLASVAV GDVRGVRSWL GTRTMVLLGE
     LTFAFYLVHY LIIQYGHRFA GGKQGYYRQW DTPAAVGLTL LAFTLALGLS AFLHFFVEKP
     VMRTLGRPRR SPDAGSTPRS EPAPSGTP