CARF_MOUSE
ID CARF_MOUSE Reviewed; 563 AA.
AC Q8BI72;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=CDKN2A-interacting protein;
DE AltName: Full=Collaborator of ARF;
GN Name=Cdkn2aip; Synonyms=Carf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION.
RX PubMed=12581788; DOI=10.1016/s0531-5565(02)00180-8;
RA Wadhwa R., Sugihara T., Hasan M.K., Duncan E.L., Taira K., Kaul S.C.;
RT "A novel putative collaborator of p19ARF.";
RL Exp. Gerontol. 38:245-252(2003).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-234, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulates DNA damage response and cell proliferation in a
CC dose-dependent manner through a number of signaling pathways involved
CC in cell proliferation, apoptosis and senescence.
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SUBUNIT: Interacts with CDKN2A/p14ARF, p53/TP53 and MDM2. Interacts
CC with CHEK2 and MAPK3. Interacts with XRN2.
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- PTM: May be ubiquitinated. {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SIMILARITY: Belongs to the CARF family. {ECO:0000305}.
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DR EMBL; AK048437; BAC33336.1; -; mRNA.
DR CCDS; CCDS22299.1; -.
DR RefSeq; NP_765995.1; NM_172407.3.
DR AlphaFoldDB; Q8BI72; -.
DR SMR; Q8BI72; -.
DR BioGRID; 214348; 23.
DR IntAct; Q8BI72; 17.
DR STRING; 10090.ENSMUSP00000043713; -.
DR iPTMnet; Q8BI72; -.
DR PhosphoSitePlus; Q8BI72; -.
DR EPD; Q8BI72; -.
DR jPOST; Q8BI72; -.
DR MaxQB; Q8BI72; -.
DR PaxDb; Q8BI72; -.
DR PeptideAtlas; Q8BI72; -.
DR PRIDE; Q8BI72; -.
DR ProteomicsDB; 265435; -.
DR Antibodypedia; 17295; 227 antibodies from 32 providers.
DR DNASU; 70925; -.
DR Ensembl; ENSMUST00000212175; ENSMUSP00000148441; ENSMUSG00000038069.
DR GeneID; 70925; -.
DR KEGG; mmu:70925; -.
DR UCSC; uc009lrh.2; mouse.
DR CTD; 55602; -.
DR MGI; MGI:1918175; Cdkn2aip.
DR VEuPathDB; HostDB:ENSMUSG00000038069; -.
DR eggNOG; ENOG502S4FT; Eukaryota.
DR GeneTree; ENSGT00940000158376; -.
DR HOGENOM; CLU_019689_0_0_1; -.
DR InParanoid; Q8BI72; -.
DR OMA; CRYPPQV; -.
DR OrthoDB; 1524198at2759; -.
DR PhylomeDB; Q8BI72; -.
DR TreeFam; TF333807; -.
DR BioGRID-ORCS; 70925; 6 hits in 73 CRISPR screens.
DR ChiTaRS; Cdkn2aip; mouse.
DR PRO; PR:Q8BI72; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BI72; protein.
DR Bgee; ENSMUSG00000038069; Expressed in spermatid and 215 other tissues.
DR ExpressionAtlas; Q8BI72; baseline and differential.
DR Genevisible; Q8BI72; MM.
DR GO; GO:0001652; C:granular component; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0002039; F:p53 binding; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0030308; P:negative regulation of cell growth; ISO:MGI.
DR GO; GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
DR GO; GO:0009967; P:positive regulation of signal transduction; ISO:MGI.
DR GO; GO:0031647; P:regulation of protein stability; ISO:MGI.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR021859; XTBD.
DR Pfam; PF11952; XTBD; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS51827; XTBD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW RNA-binding; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT CHAIN 2..563
FT /note="CDKN2A-interacting protein"
FT /id="PRO_0000324340"
FT DOMAIN 19..126
FT /note="XRN2-binding (XTBD)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01171"
FT DOMAIN 445..520
FT /note="DRBM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT REGION 122..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..221
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT MOD_RES 124
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 340
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT MOD_RES 371
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT CROSSLNK 177
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1)"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
SQ SEQUENCE 563 AA; 59745 MW; 277B979F01EB43C1 CRC64;
MAQEVSEYLS QNPRVAAWVE TLRCEGETDK HWRHRREFLL RNAGDLVPAT DETADAESGA
RTRQLQQLVS FSMAWANHVF LGCRYPQKVM DKILSMAEGI KVTDAPIHTT RDELVAKVKK
RGISSSNEGV EEPSKKRAVE GKNNSSVERD HGKKSAKTDR SAAQQENSSP SRGSSTKSES
GGTSARSSSS GSHQDSATSE GDRSVCSQSS SNSSQVTAGS GKALESEAPH KRGSASFVSS
LLKSSMNSHM TQSTDNRQQS GSPKKGALEG SSGSASQSSS EIEVPLLGSS GSAEVELPLL
SCKSSSETAS SGLTSKSSSE ANISSSVSKN SSSSGSSLLT PQSSSTNPSL LTSKSTAQVA
ASLLATKSGA SLGSVSQLAA KSGSQSSTSQ LPSKSTSQAS ESSVKFACRK LTNEDIKQKQ
PFFNRLYKTV AWKLVAVGGF SPTVNHGELL NAAIEALKAT LDVFFVPLKE LADLPQNKSS
QESIVCELRC KSVYLGTGCG KSKENAKAVA SREALKLFLK KKVVVKICKR KYRGSEIEDL
VLLDEEARPV NLPPALKHPQ ELL
