ID   Y3649_ARATH             Reviewed;         588 AA.
AC   Q9LIM6; A0A1I9LS36;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=BTB/POZ domain-containing protein At3g26490;
GN   OrderedLocusNames=At3g26490; ORFNames=F20C19.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND SER-378, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. La-0;
RX   PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA   Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT   "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT   immobilized metal ion affinity chromatography and mass spectrometry.";
RL   Mol. Cell. Proteomics 2:1234-1243(2003).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND SER-378, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA   Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT   "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT   phosphorylation site database.";
RL   Plant Cell 16:2394-2405(2004).
RN   [5]
RP   DOMAIN BTB.
RX   PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA   Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA   Vierstra R.D.;
RT   "Cullins 3a and 3b assemble with members of the broad
RT   complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT   ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL   J. Biol. Chem. 280:18810-18821(2005).
CC   -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC       protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC       ubiquitination and subsequent proteasomal degradation of target
CC       proteins. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC       of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC   -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00982}.
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DR   EMBL; AP001298; BAB02210.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77166.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM65393.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM65394.1; -; Genomic_DNA.
DR   RefSeq; NP_001319646.1; NM_001338805.1.
DR   RefSeq; NP_001327366.1; NM_001338807.1.
DR   RefSeq; NP_189280.1; NM_113556.2.
DR   AlphaFoldDB; Q9LIM6; -.
DR   STRING; 3702.AT3G26490.1; -.
DR   iPTMnet; Q9LIM6; -.
DR   PaxDb; Q9LIM6; -.
DR   PRIDE; Q9LIM6; -.
DR   ProteomicsDB; 242361; -.
DR   EnsemblPlants; AT3G26490.1; AT3G26490.1; AT3G26490.
DR   EnsemblPlants; AT3G26490.3; AT3G26490.3; AT3G26490.
DR   EnsemblPlants; AT3G26490.4; AT3G26490.4; AT3G26490.
DR   GeneID; 822256; -.
DR   Gramene; AT3G26490.1; AT3G26490.1; AT3G26490.
DR   Gramene; AT3G26490.3; AT3G26490.3; AT3G26490.
DR   Gramene; AT3G26490.4; AT3G26490.4; AT3G26490.
DR   KEGG; ath:AT3G26490; -.
DR   Araport; AT3G26490; -.
DR   TAIR; locus:2079296; AT3G26490.
DR   eggNOG; ENOG502QSYM; Eukaryota.
DR   HOGENOM; CLU_005994_5_2_1; -.
DR   InParanoid; Q9LIM6; -.
DR   OMA; SSRWWGE; -.
DR   OrthoDB; 510893at2759; -.
DR   PhylomeDB; Q9LIM6; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9LIM6; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LIM6; baseline and differential.
DR   Genevisible; Q9LIM6; AT.
DR   GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR043454; NPH3/RPT2-like.
DR   InterPro; IPR027356; NPH3_dom.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR32370; PTHR32370; 1.
DR   Pfam; PF03000; NPH3; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS51649; NPH3; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..588
FT                   /note="BTB/POZ domain-containing protein At3g26490"
FT                   /id="PRO_0000409577"
FT   DOMAIN          28..99
FT                   /note="BTB"
FT   DOMAIN          218..507
FT                   /note="NPH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT   REGION          529..554
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        529..548
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         376
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:14506206,
FT                   ECO:0007744|PubMed:15308754"
FT   MOD_RES         378
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:14506206,
FT                   ECO:0007744|PubMed:15308754"
FT   MOD_RES         448
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FMF5"
SQ   SEQUENCE   588 AA;  66348 MW;  705386E36E1BE0CC CRC64;
     MKFMELGFRP DTFYTVESVR SVSSDLLNDL VIQVKSTKYL LHKFPMLSKC LRLKNLVSSQ
     ETETSQEQQV IQLVDFPGET EAFELCAKFC YGITITLCAH NVVAVRCAAE YLGMTEEVEL
     GETENLVQRL ELFLTTCVFK SWRDSYVTLQ TTKVLPLWSE DLGITNRCIE AIANGVTVSP
     GEDFSTQLET GLLRNRSRIR RDEILCNGGG GSKAESLRWW GEDLAELGLD LYRRTMVAIK
     SSHRKISPRL IGNALRIYAS KWLPSIQESS ADSNLVLESV ISLLPEEKSS VPCSFLLQLL
     KMANVMNVSH SSKMELAIKA GNQLDKATVS ELLIPLSDKS GMLYDVDVVK MMVKQFLSHI
     SPEIRPTRTR TEHRRSRSEE NINLEEIQEV RGSLSTSSSP PPLLSKVAKL VDSYLQEIAR
     DVNLTVSKFV ELAETIPDTS RICHDDLYNA IDVYLQVHKK IEKCERKRLC RILDCKKLSV
     EASKKAAQNE LLPLRVIVQI LFVEQARATL ATTRNNITTN ETAVLKRSFT TRREEGGQEE
     EERDETKPSG GFLQSTPSRF MALCAIPRQP KKLLCKLLSI SRSLSQRI