ID   Y3649_PSEAE             Reviewed;         450 AA.
AC   Q9HXY3;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Putative zinc metalloprotease PA3649;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=PA3649;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG07037.1; -; Genomic_DNA.
DR   PIR; D83188; D83188.
DR   RefSeq; NP_252339.1; NC_002516.2.
DR   RefSeq; WP_003098594.1; NZ_QZGE01000001.1.
DR   AlphaFoldDB; Q9HXY3; -.
DR   SMR; Q9HXY3; -.
DR   STRING; 287.DR97_4290; -.
DR   PaxDb; Q9HXY3; -.
DR   PRIDE; Q9HXY3; -.
DR   EnsemblBacteria; AAG07037; AAG07037; PA3649.
DR   GeneID; 880507; -.
DR   KEGG; pae:PA3649; -.
DR   PseudoCAP; PA3649; -.
DR   HOGENOM; CLU_025778_0_2_6; -.
DR   InParanoid; Q9HXY3; -.
DR   OMA; QYMVGFG; -.
DR   PhylomeDB; Q9HXY3; -.
DR   BioCyc; PAER208964:G1FZ6-3719-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008237; F:metallopeptidase activity; ISO:PseudoCAP.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IDA:PseudoCAP.
DR   Gene3D; 2.30.42.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 2.
DR   Pfam; PF13180; PDZ_2; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 2.
DR   SUPFAM; SSF50156; SSF50156; 2.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   CHAIN           1..450
FT                   /note="Putative zinc metalloprotease PA3649"
FT                   /id="PRO_0000088453"
FT   TRANSMEM        97..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..444
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          199..291
FT                   /note="PDZ"
FT   ACT_SITE        22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         21
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         25
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   450 AA;  48530 MW;  94634CA589D3EA4E CRC64;
     MSALYMIVGT LVALGVLVTF HEFGHFWVAR RCGVKVLRFS VGFGTPLVRW HDRHGTEFVV
     AAIPLGGYVK MLDEREAEVP AHLLEQSFNR KTVRQRIAIV AAGPIANFLL AILFFWVVAL
     LGSQQVRPVI GSVAPESLAA QAGLEAGQEL LAVDGEPVTG WNGVNLQLVR RLGESGTLEV
     RVQEKGSNVD STHQVRLDGW LKGEDNPDPI ASLGIRPWRP ALPPVLAELD PKGPAQAAGL
     KLGDRLQSID GIAVDDWQQV VDSVRARPGQ RVQLKVLRDG EVLDVALELA VRGEGKARSG
     YMGAGVAGTE WPAEMLREVS YGPLEAVGQA LSRTWTMSLL TLDSIKKMLL GELSVKNLSG
     PITIAKVAGA SAQSGVGDFL NFLAYLSISL GVLNLLPIPV LDGGHLLFYL VEWVRGRPLS
     ERVQAWGMQI GISLVVGVML LALVNDLSRL