CARF_RAT
ID CARF_RAT Reviewed; 570 AA.
AC Q5U2X0; Q4KM89;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=CDKN2A-interacting protein;
DE AltName: Full=Collaborator of ARF;
GN Name=Cdkn2aip; Synonyms=Carf;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Testis, and Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16641100; DOI=10.1073/pnas.0600895103;
RA Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.;
RT "Quantitative phosphoproteomics of vasopressin-sensitive renal cells:
RT regulation of aquaporin-2 phosphorylation at two sites.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).
CC -!- FUNCTION: Regulates DNA damage response and cell proliferation in a
CC dose-dependent manner through a number of signaling pathways involved
CC in cell proliferation, apoptosis and senescence.
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SUBUNIT: Interacts with CDKN2A/p14ARF, p53/TP53 and MDM2. Interacts
CC with CHEK2 and MAPK3. Interacts with XRN2.
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5U2X0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5U2X0-2; Sequence=VSP_032223, VSP_032224;
CC -!- PTM: May be ubiquitinated. {ECO:0000250|UniProtKB:Q9NXV6}.
CC -!- SIMILARITY: Belongs to the CARF family. {ECO:0000305}.
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DR EMBL; BC085832; AAH85832.1; -; mRNA.
DR EMBL; BC098694; AAH98694.1; -; mRNA.
DR RefSeq; NP_001014022.1; NM_001014000.1. [Q5U2X0-1]
DR RefSeq; XP_006253209.1; XM_006253147.3. [Q5U2X0-2]
DR AlphaFoldDB; Q5U2X0; -.
DR SMR; Q5U2X0; -.
DR STRING; 10116.ENSRNOP00000029705; -.
DR iPTMnet; Q5U2X0; -.
DR PhosphoSitePlus; Q5U2X0; -.
DR jPOST; Q5U2X0; -.
DR PaxDb; Q5U2X0; -.
DR PeptideAtlas; Q5U2X0; -.
DR PRIDE; Q5U2X0; -.
DR Ensembl; ENSRNOT00000030519; ENSRNOP00000029705; ENSRNOG00000022736. [Q5U2X0-1]
DR GeneID; 306455; -.
DR KEGG; rno:306455; -.
DR CTD; 55602; -.
DR RGD; 1305302; Cdkn2aip.
DR eggNOG; ENOG502S4FT; Eukaryota.
DR GeneTree; ENSGT00940000158376; -.
DR HOGENOM; CLU_019689_0_0_1; -.
DR InParanoid; Q5U2X0; -.
DR OMA; CRYPPQV; -.
DR OrthoDB; 1524198at2759; -.
DR PhylomeDB; Q5U2X0; -.
DR TreeFam; TF333807; -.
DR PRO; PR:Q5U2X0; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Bgee; ENSRNOG00000022736; Expressed in testis and 19 other tissues.
DR GO; GO:0001652; C:granular component; ISO:RGD.
DR GO; GO:0005730; C:nucleolus; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0002039; F:p53 binding; ISO:RGD.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0030308; P:negative regulation of cell growth; ISO:RGD.
DR GO; GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
DR GO; GO:0009967; P:positive regulation of signal transduction; ISO:RGD.
DR GO; GO:0031647; P:regulation of protein stability; ISO:RGD.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR021859; XTBD.
DR Pfam; PF11952; XTBD; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS51827; XTBD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Isopeptide bond; Nucleus;
KW Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT CHAIN 2..570
FT /note="CDKN2A-interacting protein"
FT /id="PRO_0000324341"
FT DOMAIN 19..126
FT /note="XRN2-binding (XTBD)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01171"
FT DOMAIN 452..527
FT /note="DRBM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT REGION 122..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..221
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..287
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT MOD_RES 124
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BI72"
FT MOD_RES 378
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT CROSSLNK 176
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1)"
FT /evidence="ECO:0000250|UniProtKB:Q9NXV6"
FT VAR_SEQ 118..119
FT /note="VK -> KG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032223"
FT VAR_SEQ 120..570
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032224"
SQ SEQUENCE 570 AA; 60744 MW; E31CB97382220015 CRC64;
MAQEVSEYLS QNPRVAAWVE TLRCEGETDK HWRHRREFLL RNAGDLVPAT EETADAESGA
RSRQLQQLVS FSMAWANHVF LGCRYPQKVM DKILSMAEGI KVTDAPIHTT RDELVAKVKK
RGISSSNEGV EEPSKKRSIE GKNNSSVERD HGKKSAKTDR SAQQENSSGS KGSSTKSESS
GTSARSNSGV SHQNSSTSEG DRSVCSQSSS NSSQVTSAGS GKASEPEAPD KHGSASFVSS
LLKSSLNSHV TKSTDSRQHS GSPRKNALEG SSVSVSQSSS EIEVPLLGSS GSSEVELPLL
SCKSSSETAS SGLTTKASSE ANISSSVSKN SSSSGTSLLM PKSSSTNTSL LTSQVAASLL
ASKSSSQSSG SVASKSTSLG SMSQLASKSS SQSSTSQLPS KSTSQSSESS VKFTCRKLTN
EDIKQKQPFF NRLYKTVAWK LVAVGGFSPN VNHGELLNAA IEALKATLDV FFVPLKELAD
LPQNKSSQES IVCELRCKSV YLGTGCGKSK ENAKAVASRE ALKLFLKKKV VVKICKRKYR
GNEIEDLVLL DEESRPVNLP PALKHPQELL
