CARH_THET8
ID CARH_THET8 Reviewed; 285 AA.
AC Q53W62;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=HTH-type transcriptional repressor CarH;
GN Name=carH; OrderedLocusNames=TTHB100;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OG Plasmid pTT27.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, DNA-BINDING, AND ACTIVITY REGULATION.
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=21502508; DOI=10.1073/pnas.1018972108;
RA Ortiz-Guerrero J.M., Polanco M.C., Murillo F.J., Padmanabhan S.,
RA Elias-Arnanz M.;
RT "Light-dependent gene regulation by a coenzyme B12-based photoreceptor.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:7565-7570(2011).
RN [3]
RP ACTIVITY REGULATION, DNA-BINDING, AND SUBUNIT.
RX PubMed=23512413; DOI=10.1007/s00249-013-0897-x;
RA Diez A.I., Ortiz-Guerrero J.M., Ortega A., Elias-Arnanz M., Padmanabhan S.,
RA Garcia de la Torre J.;
RT "Analytical ultracentrifugation studies of oligomerization and DNA-binding
RT of TtCarH, a Thermus thermophilus coenzyme B12-based photosensory
RT regulator.";
RL Eur. Biophys. J. 42:463-476(2013).
CC -!- FUNCTION: Negative regulator of genes involved in carotenoid
CC biosynthesis. {ECO:0000269|PubMed:21502508}.
CC -!- ACTIVITY REGULATION: Requires cobalamin (vitamin B12) for repressor
CC activity. In the dark, binding of cobalamin to CarH induces its
CC oligomerization, which enhances binding to the DNA and repressor
CC activity. Light causes cobalamin photolysis and disruption of the
CC cobalamin-CarH complex, which decreases interaction with DNA and allows
CC transcription of the target genes. {ECO:0000269|PubMed:21502508,
CC ECO:0000269|PubMed:23512413}.
CC -!- SUBUNIT: Homotetramer in the dark in the presence of cobalamin. Monomer
CC in the light or in the absence of cobalamin.
CC {ECO:0000269|PubMed:23512413}.
CC -!- DOMAIN: The N-terminal region interacts with DNA, and the C-terminal
CC region is involved in oligomerization and binding of cobalamin.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CarA/CarH B12-binding photoregulator family.
CC {ECO:0000305}.
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DR EMBL; AP008227; BAD71896.1; -; Genomic_DNA.
DR RefSeq; WP_011229194.1; NC_006462.1.
DR RefSeq; YP_145339.1; NC_006462.1.
DR AlphaFoldDB; Q53W62; -.
DR SMR; Q53W62; -.
DR EnsemblBacteria; BAD71896; BAD71896; BAD71896.
DR GeneID; 3169555; -.
DR KEGG; ttj:TTHB100; -.
DR PATRIC; fig|300852.9.peg.2043; -.
DR HOGENOM; CLU_045945_3_0_0; -.
DR OMA; IWEKRYN; -.
DR PhylomeDB; Q53W62; -.
DR Proteomes; UP000000532; Plasmid pTT27.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1240.10; -; 1.
DR InterPro; IPR003759; Cbl-bd_cap.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR000551; MerR-type_HTH_dom.
DR InterPro; IPR036594; Meth_synthase_dom.
DR Pfam; PF02607; B12-binding_2; 1.
DR Pfam; PF13411; MerR_1; 1.
DR SMART; SM00422; HTH_MERR; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
DR SUPFAM; SSF52242; SSF52242; 1.
DR PROSITE; PS50937; HTH_MERR_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; DNA-binding; Plasmid; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..285
FT /note="HTH-type transcriptional repressor CarH"
FT /id="PRO_0000429424"
FT DOMAIN 6..76
FT /note="HTH merR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT DOMAIN 164..285
FT /note="B12-binding"
FT DNA_BIND 9..28
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
SQ SEQUENCE 285 AA; 31139 MW; 075D433AFDAB1511 CRC64;
MTSSGVYTIA EVEAMTGLSA EVLRQWERRY GFPKPRRTPG GHRLYSAEDV EALKTIKRWL
EEGATPKAAI RRYLAQEVRP EDLGTGLLEA LLRGDLAGAE ALFRRGLRFW GPEGVLEHLL
LPVLREVGEA WHRGEIGVAE EHLASTFLRA RLQELLDLAG FPPGPPVLVT TPPGERHEIG
AMLAAYHLRR KGVPALYLGP DTPLPDLRAL ARRLGAGTVV LSAVLSEPLR ALPDGALKDL
APRVFLGGQG AGPEEARRLG AEYMEDLKGL AEALWLPRGP EKEAI
