CARL3_MOUSE
ID CARL3_MOUSE Reviewed; 1375 AA.
AC Q3UFQ8; Q6KAP3;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Capping protein, Arp2/3 and myosin-I linker protein 3 {ECO:0000250|UniProtKB:Q8ND23};
DE AltName: Full=Capping protein regulator and myosin 1 linker protein 3;
DE AltName: Full=Leucine-rich repeat-containing protein 16B {ECO:0000312|MGI:MGI:2448573};
GN Name=Carmil3; Synonyms=Lrrc16b {ECO:0000312|MGI:MGI:2448573};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-898.
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8ND23}. Cell
CC membrane {ECO:0000250|UniProtKB:Q8ND23}.
CC -!- DOMAIN: The C-terminus is necessary for localization to the cell
CC membrane. {ECO:0000250|UniProtKB:Q8ND23}.
CC -!- SIMILARITY: Belongs to the CARMIL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD21414.1; Type=Miscellaneous discrepancy; Note=The sequence differs from that shown because it seems to be derived from a pre-mRNA.; Evidence={ECO:0000305};
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DR EMBL; AK131164; BAD21414.1; ALT_SEQ; Transcribed_RNA.
DR EMBL; AK148355; BAE28502.1; -; mRNA.
DR CCDS; CCDS36930.1; -.
DR RefSeq; NP_001019816.1; NM_001024645.1.
DR AlphaFoldDB; Q3UFQ8; -.
DR SMR; Q3UFQ8; -.
DR BioGRID; 234547; 3.
DR IntAct; Q3UFQ8; 2.
DR MINT; Q3UFQ8; -.
DR STRING; 10090.ENSMUSP00000075587; -.
DR iPTMnet; Q3UFQ8; -.
DR PhosphoSitePlus; Q3UFQ8; -.
DR MaxQB; Q3UFQ8; -.
DR PaxDb; Q3UFQ8; -.
DR PRIDE; Q3UFQ8; -.
DR ProteomicsDB; 265436; -.
DR Antibodypedia; 22575; 40 antibodies from 12 providers.
DR DNASU; 268747; -.
DR Ensembl; ENSMUST00000076236; ENSMUSP00000075587; ENSMUSG00000022211.
DR GeneID; 268747; -.
DR KEGG; mmu:268747; -.
DR UCSC; uc007tyo.1; mouse.
DR CTD; 90668; -.
DR MGI; MGI:2448573; Carmil3.
DR VEuPathDB; HostDB:ENSMUSG00000022211; -.
DR eggNOG; KOG4242; Eukaryota.
DR GeneTree; ENSGT00940000157990; -.
DR HOGENOM; CLU_003119_3_2_1; -.
DR InParanoid; Q3UFQ8; -.
DR OMA; NHEHEET; -.
DR OrthoDB; 208951at2759; -.
DR PhylomeDB; Q3UFQ8; -.
DR TreeFam; TF316381; -.
DR BioGRID-ORCS; 268747; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Lrrc16b; mouse.
DR PRO; PR:Q3UFQ8; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q3UFQ8; protein.
DR Bgee; ENSMUSG00000022211; Expressed in embryonic brain and 156 other tissues.
DR ExpressionAtlas; Q3UFQ8; baseline and differential.
DR Genevisible; Q3UFQ8; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0030027; C:lamellipodium; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:2000249; P:regulation of actin cytoskeleton reorganization; IBA:GO_Central.
DR GO; GO:0034315; P:regulation of Arp2/3 complex-mediated actin nucleation; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR029766; CARMIL3.
DR InterPro; IPR031943; CARMIL_C.
DR InterPro; IPR041245; CARMIL_PH.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR PANTHER; PTHR24112:SF43; PTHR24112:SF43; 1.
DR Pfam; PF17888; Carm_PH; 1.
DR Pfam; PF16000; CARMIL_C; 1.
DR Pfam; PF13516; LRR_6; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Leucine-rich repeat; Membrane;
KW Reference proteome; Repeat.
FT CHAIN 1..1375
FT /note="Capping protein, Arp2/3 and myosin-I linker protein
FT 3"
FT /id="PRO_0000324609"
FT REPEAT 244..264
FT /note="LRR 1"
FT REPEAT 274..295
FT /note="LRR 2"
FT REPEAT 303..323
FT /note="LRR 3"
FT REPEAT 335..357
FT /note="LRR 4"
FT REPEAT 365..386
FT /note="LRR 5"
FT REPEAT 392..413
FT /note="LRR 6"
FT REPEAT 424..444
FT /note="LRR 7"
FT REPEAT 455..475
FT /note="LRR 8"
FT REPEAT 482..501
FT /note="LRR 9"
FT REPEAT 509..530
FT /note="LRR 10"
FT REGION 124..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 864..901
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 969..1375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 864..881
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 882..897
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 979..995
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1006..1020
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1074..1099
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1168..1190
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1231..1246
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1344..1359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1375 AA; 150416 MW; A6963D11222F14C2 CRC64;
MAKASVELTR ELQDSIRRCL SQGAVLQQHR VKLETKPKKF EDRVLALTSW RLHLFPLKVP
AKVESSFNVL EIRAFNTLSQ NQILVETERG TVSMRLPSAE SVDQVTRHVS SALSKVCPGP
GCLIRRGNAD TPEGPRDTSP NSETSTSTTH SVCGGFSETY AALCDYNGLH CREEVQWDVD
TIYHAEDNRE FNLLDFSHLE SRDLALMVAA LAYNQWFTKL YCKDLRLGSE VLEQVLHTLS
KSGSLEELVL DNAGLKTDFV QKLAGVFGEN GSCVLHALIL SHNPIEDKGF LSLSQQLLCF
PTGLTKLCLA KTAISPRGLQ ALGQTFGANP AFASSLRYLD LSKNPGLLAT DEANALYSFL
AQPNALVHLD LSGTDCAVDM LLGALLHGCC SHLTYLNLAR NSCSHRKGRE APPAFKQFFS
SVYTLSHVNL SATRLPLEAL RALLQGLSLN SHLSDLHLDL SSCELRSAGA QALQEQLGAV
TCIGSLDLSD NGFDSDLLTL VPALGKNKSL KHLFLGKNFN VKAKTLEEIL HKLVQLIQEE
DCSLQSLSVA DSRLKLRTSI LINALGSNTC LAKVDLSGNG MEDIGAKMLS KALQINSSLR
TILWDRNNTS ALGFLDIARA LESNHTLRFM SFPVSDISQA YRSAPERTED VWQKIQWCLV
RNNHSQTCPQ EQAFRLQQGL VTSSAEQMLQ RLCGRVQEEV RALRLCPLEP VQDELLYARD
LIKDAKNSRA LFPSLYELGH VLANDGPVRQ RLESVASEVS KAVDKELQVI LESMVSLTQE
LCPVAMRVAE GHNKMLSNVA ERVTVPRNFI RGALLEQAGQ DIQNKLDEVK LSVVTYLTNS
IVDEILQELY HSHKSLARHL TQLRTLSDPP GGASQGQDPS SRGRGRNHDH EETDDELGTN
IDTMAIKKQK RCRKIRPVSA FISGSPQDME SQLGSLGIPP GWFSGLGASQ TTASGSWEGL
SELPTHGYKL RHQTQGRPRP PRTTPPGPGR PSVPVPGPRQ ENGMATRLDE GLEDFFSRRV
MDESSSYPRT LRTMRPGLSE PPLPPLQKKR RRGLFHFRRP RSFKGDRGPG SPTAGLLLPP
PPPPPPTQES PPSPDPPSLG NNSSPCWSPE EESSLLPGFG GARGSSFCRK MGTERLEAGE
GAPAPGTAQQ PRVHGGVALP GLGRTKGWSF DGKREGTDPD QEDSTQAWQK RRSSDDAGPG
AWKPPPPPQS SKPSFSAMRR AEATWHIAEE SAANHSCQSP SPASQDGDEE KQGALFPERM
VPTRNAKLQE PPIGPRPPKP VAVPRGRRAP QVPGGREETE SSSAAPGANK PRLRLGSQQD
QEEPEGQGPT DQGRRTAPLK PKRTRRAQSC DKLEPDRRQP PDPTGVCGTS EPGTD
