Y370_MYCGE
ID Y370_MYCGE Reviewed; 323 AA.
AC P47610;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase MG370;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=MG370;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-199.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; L43967; AAC71597.1; -; Genomic_DNA.
DR EMBL; U02220; AAA03374.1; -; Genomic_DNA.
DR PIR; I64240; I64240.
DR RefSeq; WP_010869454.1; NC_000908.2.
DR AlphaFoldDB; P47610; -.
DR SMR; P47610; -.
DR STRING; 243273.MG_370; -.
DR EnsemblBacteria; AAC71597; AAC71597; MG_370.
DR KEGG; mge:MG_370; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_1_0_14; -.
DR OMA; HAFLVKF; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding.
FT CHAIN 1..323
FT /note="Uncharacterized RNA pseudouridine synthase MG370"
FT /id="PRO_0000162741"
FT DOMAIN 16..95
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 148
FT /evidence="ECO:0000250"
SQ SEQUENCE 323 AA; 37948 MW; 0A0897B8C7367FB8 CRC64;
MRIANKFLVP KESENQRIDQ FCLKILPLIK RSDFFKYLRL GKVLLNKTKP QVNTRLKTKD
EIAFLFDINP YLQTNNDYLS LDLVKDKLKI IFEDENIIVV DKPTGIVCQP DKKHSIINLS
NMLLKHCGYR QFDSNKLNFY PQFAHRIDRN TSGIVIGAKT NKALKELNKV FKNNHLTKRY
KGLVFGQFNH LGLQTAYWKK DNNNGIVTVK WKPFPEAKKI STFFENSSYI AQKDMSLITI
RLISGRTHQI RACLNLFSNQ LVGDKKYSLI QFKNRNNKYK HQALHAYELV FPKLETSKFP
ILTNYSLMQF KSKIVPWFEY LIQ
