Y3725_ARATH
ID Y3725_ARATH Reviewed; 620 AA.
AC Q0WTB4; Q9M2Y5;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=GTP-binding protein At3g49725, chloroplastic;
DE Flags: Precursor;
GN OrderedLocusNames=At3g49725; ORFNames=T16K5.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU01042};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- DOMAIN: In contrast to other GTP-binding proteins, this family is
CC characterized by a circular permutation of the GTPase motifs described
CC by a G4-G1-G3 pattern.
CC -!- SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase
CC superfamily. HflX GTPase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01042}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB66911.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At3g49725 and At3g49730.; Evidence={ECO:0000305};
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DR EMBL; AL132965; CAB66911.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78582.1; -; Genomic_DNA.
DR EMBL; AK227647; BAE99634.1; -; mRNA.
DR PIR; T46039; T46039.
DR RefSeq; NP_001154669.1; NM_001161197.2.
DR AlphaFoldDB; Q0WTB4; -.
DR SMR; Q0WTB4; -.
DR STRING; 3702.AT3G49725.1; -.
DR PaxDb; Q0WTB4; -.
DR PRIDE; Q0WTB4; -.
DR ProteomicsDB; 228612; -.
DR EnsemblPlants; AT3G49725.1; AT3G49725.1; AT3G49725.
DR GeneID; 7922380; -.
DR Gramene; AT3G49725.1; AT3G49725.1; AT3G49725.
DR KEGG; ath:AT3G49725; -.
DR Araport; AT3G49725; -.
DR TAIR; locus:5019474783; AT3G49725.
DR eggNOG; KOG0410; Eukaryota.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_019597_6_0_1; -.
DR OMA; DQRDGYF; -.
DR OrthoDB; 1121388at2759; -.
DR PhylomeDB; Q0WTB4; -.
DR PRO; PR:Q0WTB4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q0WTB4; baseline and differential.
DR Genevisible; Q0WTB4; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR CDD; cd01878; HflX; 1.
DR Gene3D; 3.40.50.11060; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR030394; G_HFLX_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR032305; GTP-bd_M.
DR InterPro; IPR016496; GTPase_HflX.
DR InterPro; IPR025121; GTPase_HflX_N.
DR InterPro; IPR042108; GTPase_HflX_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10229; PTHR10229; 1.
DR Pfam; PF16360; GTP-bdg_M; 1.
DR Pfam; PF13167; GTP-bdg_N; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03156; GTP_HflX; 1.
DR PROSITE; PS51705; G_HFLX; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 66..620
FT /note="GTP-binding protein At3g49725, chloroplastic"
FT /id="PRO_0000355994"
FT DOMAIN 346..585
FT /note="Hflx-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT REGION 57..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 478..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 597..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..95
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..502
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 352..359
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 359
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 377..381
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 379
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 399..402
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 468..471
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT BINDING 563..565
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01042"
FT CONFLICT 368
FT /note="T -> A (in Ref. 3; BAE99634)"
FT /evidence="ECO:0000305"
FT CONFLICT 540
FT /note="E -> G (in Ref. 3; BAE99634)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 620 AA; 69579 MW; 94F0F6ECD532C929 CRC64;
MSVTTSFGIW LNHINLDNQA SFLYNNPHFC NRRFNRISML RTVSLARNRL RSETPSSFLA
RDRLRSKTPS SSPFSSKRHT PKTSEIEEES TPKDSVLLNP KDPSSAPKLF LVQPRLAPPK
YLQAKLNEAL CLANSLEEQR YGYFESDFFD KELPSHVVVQ NPVRRSSKPR VDTYFGSGTV
DNIKCHLNAE DSKEEVDAVF VNAILTAIQQ RNLERIWAKP VLDRVGLIIE IFNAHAHTKE
AKLQAELAAL MYNKSRLVRV RGTDGRHTFG QFGEAEVVSA RGRAGSKGTG GGFVGGAGET
ELQLQRRRIS DRRIRLLSQI KEAQRTRLLQ RAGRKKRVGL EGESSGTIAV VGYTNAGKST
LISALTKTAL YCNERLFATL DPTLKSAHLP SGNFVLLSDT VGFISDLPIQ LVKAFQSTLE
EVVEADLLLH VVDSTAPNIE EHRSTVLHVL NQIGVPEEKL QNMIEVWNKI DYEEDEVEEE
KYLDDGEGVG EEDEDEADLK AEETVDASEA TVDEDQIQNG DGDDADGWLL SEDENADDPE
FWKVPEVAKV DAANKKGPDV RVSALTGVGL KELLYLIDDK MKEKKLKSPT IVERSELHKR
KWRPPRNDDE EERLIPLDQR
