ID   Y3740_MYCTO             Reviewed;         448 AA.
AC   P9WKA4; L0TF41; O69707;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT3848;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT3848;
GN   OrderedLocusNames=MT3848;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK48212.1; -; Genomic_DNA.
DR   PIR; E70798; E70798.
DR   RefSeq; WP_003900746.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKA4; -.
DR   SMR; P9WKA4; -.
DR   EnsemblBacteria; AAK48212; AAK48212; MT3848.
DR   GeneID; 45427739; -.
DR   KEGG; mtc:MT3848; -.
DR   PATRIC; fig|83331.31.peg.4141; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..448
FT                   /note="Putative diacyglycerol O-acyltransferase MT3848"
FT                   /id="PRO_0000427688"
FT   ACT_SITE        136
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   448 AA;  48353 MW;  5F386AA865E7AD7A CRC64;
     MSPIDALFLS AESREHPLHV GALQLFEPPA GAGRGFVRET YQAMLQCREI APLFRKRPTS
     LHGALINLGW STDADVDLGY HARRSALPAP GRVRELLELT SRLHSNLLDR HRPLWETHVI
     EGLRDGRFAI YSKMHHALVD GVSGLTLMRQ PMTTDPIEGK LRTAWSPATQ HTAIKRRRGR
     LQQLGGMLGS VAGLAPSTLR LARSALIEQQ LTLPFGAPHT MLNVAVGGAR RCAAQSWPLD
     RVKAVKDAAG VSLNDVVLAM CAGALREYLD DNDALPDTPL VAMVPVSLRT DRDSVGGNMV
     GAVLCNLATH LDDPADRLNA IHASMRGNKN VLSQLPRAQA LAVSLLLLSP AALNTLPGLA
     KATPPPFNVC ISNVPGAREP LYFNGARMVG NYPMSLVLDG QALNITLTST ADSLDFGVVG
     CRRSVPHVQR VLSHLETSLK ELERAVGL