Y3740_MYCTU
ID Y3740_MYCTU Reviewed; 448 AA.
AC P9WKA5; L0TF41; O69707;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Putative diacyglycerol O-acyltransferase Rv3740c;
DE EC=2.3.1.20 {ECO:0000269|PubMed:15262939};
DE AltName: Full=Putative triacylglycerol synthase Rv3740c;
GN OrderedLocusNames=Rv3740c; ORFNames=MTV025.088c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP EXPRESSION IN E.COLI, CATALYTIC ACTIVITY, INDUCTION BY HYPOXIA, AND BY
RP NITRIC OXIDE (NO).
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15262939; DOI=10.1128/jb.186.15.5017-5030.2004;
RA Daniel J., Deb C., Dubey V.S., Sirakova T.D., Abomoelak B., Morbidoni H.R.,
RA Kolattukudy P.E.;
RT "Induction of a novel class of diacylglycerol acyltransferases and
RT triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into
RT a dormancy-like state in culture.";
RL J. Bacteriol. 186:5017-5030(2004).
RN [3]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
CC -!- FUNCTION: Upon expression in E.coli has a weak triacylglycerol synthase
CC function, making triacylglycerol (TG) from diolein and long-chain fatty
CC acyl-CoA. Also functions weakly as a wax synthase, as it incorporates
CC palmityl alcohol into wax esters in the presence of palmitoyl-CoA.
CC {ECO:0000269|PubMed:15262939}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000269|PubMed:15262939};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + di-(9Z)-octadecenoylglycerol = 1,2,3-
CC tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:45780,
CC ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75945; Evidence={ECO:0000269|PubMed:15262939};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45781;
CC Evidence={ECO:0000269|PubMed:15262939};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecan-1-ol + hexadecanoyl-CoA = CoA + hexadecanyl
CC hexadecanoate; Xref=Rhea:RHEA:38167, ChEBI:CHEBI:16125,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75584;
CC Evidence={ECO:0000269|PubMed:15262939};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38168;
CC Evidence={ECO:0000269|PubMed:15262939};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- INDUCTION: A possible member of the dormancy regulon. Induced in
CC response to reduced oxygen tension (hypoxia) and low levels of nitric
CC oxide (NO). It is hoped that this regulon will give insight into the
CC latent, or dormant phase of infection. {ECO:0000269|PubMed:15262939}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP46567.1; -; Genomic_DNA.
DR PIR; E70798; E70798.
DR RefSeq; NP_218257.1; NC_000962.3.
DR RefSeq; WP_003900746.1; NZ_NVQJ01000009.1.
DR AlphaFoldDB; P9WKA5; -.
DR SMR; P9WKA5; -.
DR STRING; 83332.Rv3740c; -.
DR SwissLipids; SLP:000001152; -.
DR PaxDb; P9WKA5; -.
DR DNASU; 885781; -.
DR GeneID; 45427739; -.
DR GeneID; 885781; -.
DR KEGG; mtu:Rv3740c; -.
DR PATRIC; fig|83332.111.peg.4161; -.
DR TubercuList; Rv3740c; -.
DR eggNOG; COG1020; Bacteria.
DR OMA; VIHASMR; -.
DR PhylomeDB; P9WKA5; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IDA:MTBBASE.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0001666; P:response to hypoxia; IBA:GO_Central.
DR GO; GO:0071731; P:response to nitric oxide; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR GO; GO:0010025; P:wax biosynthetic process; IDA:MTBBASE.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; PTHR31650; 1.
DR Pfam; PF03007; WES_acyltransf; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW Reference proteome; Transferase.
FT CHAIN 1..448
FT /note="Putative diacyglycerol O-acyltransferase Rv3740c"
FT /id="PRO_0000222923"
FT ACT_SITE 136
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 448 AA; 48353 MW; 5F386AA865E7AD7A CRC64;
MSPIDALFLS AESREHPLHV GALQLFEPPA GAGRGFVRET YQAMLQCREI APLFRKRPTS
LHGALINLGW STDADVDLGY HARRSALPAP GRVRELLELT SRLHSNLLDR HRPLWETHVI
EGLRDGRFAI YSKMHHALVD GVSGLTLMRQ PMTTDPIEGK LRTAWSPATQ HTAIKRRRGR
LQQLGGMLGS VAGLAPSTLR LARSALIEQQ LTLPFGAPHT MLNVAVGGAR RCAAQSWPLD
RVKAVKDAAG VSLNDVVLAM CAGALREYLD DNDALPDTPL VAMVPVSLRT DRDSVGGNMV
GAVLCNLATH LDDPADRLNA IHASMRGNKN VLSQLPRAQA LAVSLLLLSP AALNTLPGLA
KATPPPFNVC ISNVPGAREP LYFNGARMVG NYPMSLVLDG QALNITLTST ADSLDFGVVG
CRRSVPHVQR VLSHLETSLK ELERAVGL
