ID   Y3761_MYCBO             Reviewed;         454 AA.
AC   P67211; A0A1R3Y505; O69701; X2BPF5;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase Mb3761c;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase Mb3761c;
GN   OrderedLocusNames=BQ2027_MB3761C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIU02390.1; -; Genomic_DNA.
DR   RefSeq; NP_857399.1; NC_002945.3.
DR   RefSeq; WP_003420440.1; NC_002945.4.
DR   AlphaFoldDB; P67211; -.
DR   SMR; P67211; -.
DR   EnsemblBacteria; SIU02390; SIU02390; BQ2027_MB3761C.
DR   PATRIC; fig|233413.5.peg.4116; -.
DR   OMA; FQPTFRK; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..454
FT                   /note="Putative diacyglycerol O-acyltransferase Mb3761c"
FT                   /id="PRO_0000222922"
FT   ACT_SITE        139
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   454 AA;  49304 MW;  11CD02FA1BEB6ACB CRC64;
     MDLMMPNDSM FLFIESREHP MHVGGLSLFE PPQGAGPEFV REFTERLVAN DEFQPMFRKH
     PATIGGGIAR VAWAYDDDID IDYHVRRSAL PSPGRVRDLL ELTSRLHTSL LDRHRPLWEL
     HVVEGLNDGR FAMYTKMHHA LIDGVSAMKL AQRTLSADPD DAEVRAIWNL PPRPRTRPPS
     DGSSLLDALF KMAGSVVGLA PSTLKLARAA LLEQQLTLPF AAPHSMFNVK VGGARRCAAQ
     SWSLDRIKSV KQAAGVTVND AVLAMCAGAL RYYLIERNAL PDRPLIAMVP VSLRSKEDAD
     AGGNLVGSVL CNLATHVDDP AQRIQTISAS MDGNKKVLSE LPQLQVLALS ALNMAPLTLA
     GVPGFLSAVP PPFNIVISNV PGPVDPLYYG TARLDGSYPL SNIPDGQALN ITLVNNAGNL
     DFGLVGCRRS VPHLQRLLAH LESSLKDLEQ AVGI