Y387_HAEIN
ID Y387_HAEIN Reviewed; 640 AA.
AC P44680;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable ATP-dependent DNA helicase HI_0387 {ECO:0000305};
DE EC=3.6.4.12 {ECO:0000250|UniProtKB:P27296};
GN OrderedLocusNames=HI_0387;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000250|UniProtKB:P27296};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250|UniProtKB:P27296};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250|UniProtKB:P27296};
CC -!- SIMILARITY: Belongs to the helicase family. DinG subfamily.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22045.1; -; Genomic_DNA.
DR PIR; A64065; A64065.
DR RefSeq; NP_438548.1; NC_000907.1.
DR RefSeq; WP_005693783.1; NC_000907.1.
DR AlphaFoldDB; P44680; -.
DR SMR; P44680; -.
DR STRING; 71421.HI_0387; -.
DR PRIDE; P44680; -.
DR EnsemblBacteria; AAC22045; AAC22045; HI_0387.
DR KEGG; hin:HI_0387; -.
DR PATRIC; fig|71421.8.peg.405; -.
DR eggNOG; COG1199; Bacteria.
DR HOGENOM; CLU_012117_2_0_6; -.
DR OMA; VVTNHAM; -.
DR PhylomeDB; P44680; -.
DR BioCyc; HINF71421:G1GJ1-402-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR006555; ATP-dep_Helicase_C.
DR InterPro; IPR010614; DEAD_2.
DR InterPro; IPR045028; DinG/Rad3-like.
DR InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11472; PTHR11472; 2.
DR Pfam; PF06733; DEAD_2; 1.
DR Pfam; PF13307; Helicase_C_2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00491; HELICc2; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA-binding; Helicase; Hydrolase; Iron; Iron-sulfur;
KW Metal-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..640
FT /note="Probable ATP-dependent DNA helicase HI_0387"
FT /id="PRO_0000102007"
FT DOMAIN 16..278
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 458..634
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 125..128
FT /note="DEAH box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOTIF 231..234
FT /note="DEAH box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT BINDING 51..58
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT BINDING 114
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P27296"
FT BINDING 174
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P27296"
FT BINDING 179
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P27296"
FT BINDING 185
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P27296"
SQ SEQUENCE 640 AA; 71531 MW; EC659D19C2F23D3C CRC64;
MDYENQIANI FSLNGELSQN IKGFRPRAEQ LEMAYAVGKA IQNKSSLVIE AGTGTGKTFA
YLAPALVFGK KTIISTGSKN LQDQLFNRDL PAIKKALNFT GKIALLKGRA NYLCLERLDQ
VIAQGVLGDK SVLAELSKVR KWNNSTKTGD FTECIELAED SPIIPQLTST AESCLGTDCP
NYSECYVASA RKKALNADLV VVNHHLFFAD MAVKESGFGE LIPNAEVIIF DEAHQLPDIA
SQYFGQSLTS RQLFDLCKDI NIVYRTELKD MQQLGTTSDT LLKVVQDFRL LLGNGSNVRG
NWRELYTQSA VKKAFELLQE KIDFLSEVIK LALGRSQTLD SIFERVESIK IQLKRLSETN
IVGYCYWYEG NGRQFGLHIT PLTVADKFGA QLEAKEAAWI FTSATLEVGG TFNHFCQRLG
IENATQKILY SPFNYPEQSL LCVPRYLPNT NQMNTLNSLG EILLPVIEAN KGRCFVLCTS
YSMMRGLAEY FREKSHLSIL LQGETSKGKL LEQFIKETHS VLVATSSFWE GVDVRGDALS
LVIIDKLPFT APDEPLLKAR IEDCRLQGGD PFNDIQIPEA VITLKQGVGR LIRDVTDRGV
VIICDNRLVM RNYGETFLKS LPNSSRTRDL NKVIQFLQNK
