CARP_IRPLA
ID CARP_IRPLA Reviewed; 340 AA.
AC P17576;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Polyporopepsin;
DE EC=3.4.23.29;
DE AltName: Full=Aspartic proteinase;
OS Irpex lacteus (Milk-white toothed polypore) (Polyporus tulipiferae).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Irpicaceae; Irpex.
OX NCBI_TaxID=5319;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 1-24.
RA Kobayashi H., Sekibata S., Shibuya H., Yoshida S., Kusakabe I.,
RA Murakami K.;
RT "Cloning and sequence analysis of cDNA for Irpex lacteus aspartic
RT proteinase.";
RL Agric. Biol. Chem. 53:1927-1933(1989).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Milk clotting activity, broad specificity, but fails to cleave
CC 15-Leu-|-Tyr-16 or 16-Tyr-|-Leu-17 of insulin B chain.; EC=3.4.23.29;
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR EMBL; D00589; BAA00467.1; -; mRNA.
DR PIR; JU0057; PEIKL.
DR PDB; 1WKR; X-ray; 1.30 A; A=1-340.
DR PDBsum; 1WKR; -.
DR AlphaFoldDB; P17576; -.
DR SMR; P17576; -.
DR MEROPS; A01.019; -.
DR EvolutionaryTrace; P17576; -.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd05471; pepsin_like; 1.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR034164; Pepsin-like_dom.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF00026; Asp; 1.
DR PRINTS; PR00792; PEPSIN.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 2.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aspartyl protease; Direct protein sequencing; Glycoprotein;
KW Hydrolase; Protease.
FT CHAIN 1..340
FT /note="Polyporopepsin"
FT /id="PRO_0000199512"
FT DOMAIN 14..330
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT ACT_SITE 32
FT ACT_SITE 212
FT CARBOHYD 192
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 238
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT STRAND 3..9
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 14..20
FT /evidence="ECO:0007829|PDB:1WKR"
FT TURN 21..24
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 25..32
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 53..62
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 67..79
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 82..97
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 110..113
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 116..119
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 128..134
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 137..146
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 151..164
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 167..169
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 175..178
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 185..187
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 188..197
FT /evidence="ECO:0007829|PDB:1WKR"
FT TURN 198..200
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 201..211
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 217..220
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 222..232
FT /evidence="ECO:0007829|PDB:1WKR"
FT TURN 238..240
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 241..245
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 247..251
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 256..260
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 263..267
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 270..272
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 276..282
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 289..291
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 293..295
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 306..308
FT /evidence="ECO:0007829|PDB:1WKR"
FT HELIX 310..313
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 315..321
FT /evidence="ECO:0007829|PDB:1WKR"
FT TURN 322..325
FT /evidence="ECO:0007829|PDB:1WKR"
FT STRAND 326..331
FT /evidence="ECO:0007829|PDB:1WKR"
FT TURN 333..336
FT /evidence="ECO:0007829|PDB:1WKR"
SQ SEQUENCE 340 AA; 35051 MW; 9BAF837264D42FEF CRC64;
AAGSVPATNQ LVDYVVNVGV GSPATTYSLL VDTGSSNTWL GADKSYVKTS TSSATSDKVS
VTYGSGSFSG TEYTDTVTLG SLTIPKQSIG VASRDSGFDG VDGILGVGPV DLTVGTLSPH
TSTSIPTVTD NLFSQGTIPT NLLAVSFEPT TSESSTNGEL TFGATDSSKY TGSITYTPIT
STSPASAYWG INQTIRYGSS TSILSSTAGI VDTGTTLTLI ASDAFAKYKK ATGAVADNNT
GLLRLTTAQY ANLQSLFFTI GGQTFELTAN AQIWPRNLNT AIGGSASSVY LIVGDLGSDS
GEGLDFINGL TFLERFYSVY DTTNKRLGLA TTSFTTATSN
