ID   Y3999_BURTA             Reviewed;         270 AA.
AC   Q2T7Q0;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Probable inner membrane protein BTH_II0599;
GN   OrderedLocusNames=BTH_II0599;
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS   E264).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
RN   [2]
RP   FUNCTION (MICROBIAL INFECTION), AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=25786241; DOI=10.1371/journal.pone.0120265;
RA   Koskiniemi S., Garza-Sanchez F., Edman N., Chaudhuri S., Poole S.J.,
RA   Manoil C., Hayes C.S., Low D.A.;
RT   "Genetic analysis of the CDI pathway from Burkholderia pseudomallei
RT   1026b.";
RL   PLoS ONE 10:E0120265-E0120265(2015).
RN   [3]
RP   RECEPTOR FOR CDI TOXIN ENTRY INTO TARGET CELL CYTOPLASM (MICROBIAL
RP   INFECTION).
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=26305955; DOI=10.1073/pnas.1512124112;
RA   Willett J.L., Gucinski G.C., Fatherree J.P., Low D.A., Hayes C.S.;
RT   "Contact-dependent growth inhibition toxins exploit multiple independent
RT   cell-entry pathways.";
RL   Proc. Natl. Acad. Sci. U.S.A. 112:11341-11346(2015).
CC   -!- FUNCTION: (Microbial infection) Probably transports the toxic C-
CC       terminal region of CdiA-2 from B.pseudomallei strain 1026b across the
CC       inner membrane to the cytoplasm, where CdiA has a toxic effect.
CC       Expression in E.coli makes the bacteria sensitive to the tRNase domain
CC       of B.pseudomallei strain 1026b CdiA-2. {ECO:0000269|PubMed:26305955,
CC       ECO:0000305|PubMed:25786241}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:25786241,
CC       ECO:0000305|PubMed:26305955}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Disruption confers resistance to cellular
CC       contact-dependent growth inhibition (CDI) CdiA-2 of B.pseudomallei
CC       strain 1026b, but not to endogenous CdiA. Unchanged binding to
CC       B.pseudomallei strain 1026b inhibitor cells.
CC       {ECO:0000269|PubMed:25786241}.
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DR   EMBL; CP000085; ABC35750.1; -; Genomic_DNA.
DR   RefSeq; WP_009895708.1; NZ_CP008786.1.
DR   AlphaFoldDB; Q2T7Q0; -.
DR   PRIDE; Q2T7Q0; -.
DR   EnsemblBacteria; ABC35750; ABC35750; BTH_II0599.
DR   KEGG; bte:BTH_II0599; -.
DR   HOGENOM; CLU_072075_2_0_4; -.
DR   OMA; TYRGCFN; -.
DR   OrthoDB; 1930001at2; -.
DR   Proteomes; UP000001930; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..270
FT                   /note="Probable inner membrane protein BTH_II0599"
FT                   /id="PRO_0000446876"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        198..218
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   270 AA;  28963 MW;  8C5F4A20991E83D7 CRC64;
     MQLIEVSAKT GYVWFRQGIW LFRRNPLAFV TLFFTYLLAM MLVSLVPVIG AALPLLLIPG
     IAVGFMAACR DTIAGKQVLP TILIDGFRSY GPIVTQRLLT LGGLYIVSMA AVFACSALGD
     GGTLLKIMFG LGAENLGPDA LESPGFKIAA LIAAALYAPV AMMFWFAPVL TAWHDVPPVK
     ALFFSVVSCW RNKGAFTVYG LLWFALALGV SFGLAALMQA LGASAYALMV MMPASIVITA
     MLYCSFYATY RGCFGVQEPG APKLPNTSDR