ID   Y4024_STRAW             Reviewed;         418 AA.
AC   Q82G74;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Uncharacterized N-acetyltransferase SAV_4024 {ECO:0000255|HAMAP-Rule:MF_01812};
DE            EC=2.3.1.- {ECO:0000255|HAMAP-Rule:MF_01812};
GN   OrderedLocusNames=SAV_4024;
OS   Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC
OS   14893 / NCIMB 12804 / NRRL 8165 / MA-4680).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=227882;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M.,
RA   Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces avermitilis:
RT   deducing the ability of producing secondary metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
CC   -!- SUBUNIT: Homohexamer; trimer of dimers. {ECO:0000255|HAMAP-
CC       Rule:MF_01812}.
CC   -!- SIMILARITY: Belongs to the acetyltransferase Eis family.
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DR   EMBL; BA000030; BAC71736.1; -; Genomic_DNA.
DR   RefSeq; WP_010985453.1; NZ_JZJK01000060.1.
DR   AlphaFoldDB; Q82G74; -.
DR   SMR; Q82G74; -.
DR   STRING; 227882.SAV_4024; -.
DR   PRIDE; Q82G74; -.
DR   EnsemblBacteria; BAC71736; BAC71736; SAVERM_4024.
DR   KEGG; sma:SAVERM_4024; -.
DR   eggNOG; COG4552; Bacteria.
DR   HOGENOM; CLU_050659_0_0_11; -.
DR   OMA; RRPWCPD; -.
DR   OrthoDB; 1497065at2; -.
DR   Proteomes; UP000000428; Chromosome.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1050.10; -; 1.
DR   HAMAP; MF_01812; Eis; 1.
DR   InterPro; IPR041380; Acetyltransf_17.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR025559; Eis_dom.
DR   InterPro; IPR000182; GNAT_dom.
DR   InterPro; IPR022902; NAcTrfase_Eis.
DR   InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR   Pfam; PF17668; Acetyltransf_17; 1.
DR   Pfam; PF13530; SCP2_2; 1.
DR   SUPFAM; SSF55718; SSF55718; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..418
FT                   /note="Uncharacterized N-acetyltransferase SAV_4024"
FT                   /id="PRO_0000220263"
FT   DOMAIN          7..158
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   ACT_SITE        418
FT                   /note="Proton acceptor; via carboxylate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   BINDING         87..89
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   BINDING         95..100
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
SQ   SEQUENCE   418 AA;  46001 MW;  724C85A9721C5BEB CRC64;
     MSRPDDIDVR PIAEAETADW IRALNTGFLR SPEVSEREVA DRSSYLVPAR TLGAFDNGRC
     VATFRSFPQE LTAVGGASVP ADAISNVTVT ATHRRRGLLT RMMAQDLAAA KERGDVVATL
     IAAEYPIYGR YGFGAATHST EWTIDVPRTG LDPRWSGPGD GGRIDLVDGE DVRKAGPELH
     ERLRRTQPGA VSRDERWWQV HTGVVRLDRS PWTEPFYAVY RSASGEVEGL VSYECDDHWG
     DAKQPQNTAK VNWLIATTPA AERALWHYLC SIDWITKVRT GWRAPDDLLP HFLPDPRAAR
     VTTHADWLWV RILDVVRALE ARTYDGSGTL VLDVVDRHGL AGGRYRLTVG PDGAVCEPTT
     RDAGLTLDVG ELAALWLGDA SAVRLAALGR VREQQEGAAS VADALLRTSG RPWCPDMF