CARS_LAVAN
ID CARS_LAVAN Reviewed; 548 AA.
AC U3LVZ7;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 11-DEC-2013, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=Beta-caryophyllene synthase {ECO:0000303|PubMed:24078339};
DE Short=LaCARS {ECO:0000303|PubMed:24078339};
DE EC=4.2.3.57 {ECO:0000269|PubMed:24078339};
DE AltName: Full=(-)-beta-caryophyllene synthase {ECO:0000305};
GN Name=CARS {ECO:0000303|PubMed:24078339};
OS Lavandula angustifolia (Lavender).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Lavandulinae;
OC Lavandula.
OX NCBI_TaxID=39329;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=24078339; DOI=10.1007/s11103-013-0131-3;
RA Jullien F., Moja S., Bony A., Legrand S., Petit C., Benabdelkader T.,
RA Poirot K., Fiorucci S., Guitton Y., Nicole F., Baudino S., Magnard J.L.;
RT "Isolation and functional characterization of a tau-cadinol synthase, a new
RT sesquiterpene synthase from Lavandula angustifolia.";
RL Plant Mol. Biol. 84:227-241(2014).
CC -!- FUNCTION: Sesquiterpene synthase that catalyzes the formation of
CC sesquiterpenes and sesquiterpenoid alcohols (PubMed:24078339). Converts
CC farnesyl diphosphate (FPP) to beta-caryophyllene (PubMed:24078339). Can
CC use geranyl diphosphate (GPP) to produce myrcene, limonene and camphene
CC (PubMed:24078339). {ECO:0000269|PubMed:24078339}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (-)-(E)-beta-caryophyllene +
CC diphosphate; Xref=Rhea:RHEA:28294, ChEBI:CHEBI:10357,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.57;
CC Evidence={ECO:0000269|PubMed:24078339};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28295;
CC Evidence={ECO:0000269|PubMed:24078339};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q40577};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:Q40577};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JX401283; AGL98419.1; -; mRNA.
DR AlphaFoldDB; U3LVZ7; -.
DR SMR; U3LVZ7; -.
DR BRENDA; 4.2.3.57; 9723.
DR UniPathway; UPA00213; -.
DR GO; GO:0080016; F:(-)-E-beta-caryophyllene synthase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:1901937; P:beta-caryophyllene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Lyase; Magnesium; Metal-binding.
FT CHAIN 1..548
FT /note="Beta-caryophyllene synthase"
FT /id="PRO_0000452464"
FT MOTIF 305..309
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:U3LW50"
FT BINDING 268
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 305
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 305
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 305
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 309
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 309
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 309
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 446
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 449
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 449
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 457
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 548 AA; 63644 MW; 402662EA323DCBA4 CRC64;
MAAPISTNNV CSDDARPVTY HPNVWSDYFL RYTSELTEIS VAKKEEHERQ KEAIRNLLLQ
TRDDSTLKLE LVDAIQRLGI GYHFEEEIHN SLRNIYDTNP IYNAEDDNLR VAALRFRLIR
QQGFPAPCDV FRKFVDEEGE FKSWVSNDVE GLLNLYEASN FAVHGEEILE KALEFCSLRL
EFLTQGMTNS LSMRVKEALK IPISKTLTRL GARKFMSMYQ EDESHNETLL NFAKLDFNLV
QKIHQKELNQ ITRWWKELDF GKNLPFARDR PVECYFWIVG VYFEPRYGIA RTLLTKIIYL
ASVLDDIYDV YGTLAELTIF TQIIRRWDSD AMDQLPPYMR IYCKALFDVY VEMEEEMGKI
RKSYAVEYAK KEMKRLAEMY FQEAQWAFSK YKPTMKEYLK VALISSGYMM MTINSLTTIE
DLITEEEFNW ILSEPRILRA SLTITRLMDD LAGYGTEGKM SAVHYYMAEN GVSEGEAFKE
VSGIIKSAWK DVNAECVEPR AASTTILRCV VDFTRVIVLL YSDEDAYGNS QTKTKDLIKS
VLVDPLII
