CARTF_MOUSE
ID CARTF_MOUSE Reviewed; 689 AA.
AC Q8VHI4; B2RRZ9;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Calcium-responsive transcription factor;
DE AltName: Full=Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein;
DE AltName: Full=Calcium-response factor;
DE Short=CaRF;
GN Name=Carf; Synonyms=Als2cr8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=11832226; DOI=10.1016/s0896-6273(01)00561-x;
RA Tao X., West A.E., Chen W.G., Corfas G., Greenberg M.E.;
RT "A calcium-responsive transcription factor, CaRF, that regulates neuronal
RT activity-dependent expression of BDNF.";
RL Neuron 33:383-395(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Acts as a transcriptional activator that mediates the
CC calcium- and neuron-selective induction of BDNF exon III transcription.
CC Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3'
CC sequence (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11832226}.
CC -!- TISSUE SPECIFICITY: Highly expressed in brain and testis.
CC {ECO:0000269|PubMed:11832226}.
CC -!- DOMAIN: The N-terminus is necessary for DNA-binding. The C-terminus is
CC necessary for transcriptional activation.
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DR EMBL; AF454947; AAL62331.1; -; mRNA.
DR EMBL; BC138644; AAI38645.1; -; mRNA.
DR EMBL; BC138645; AAI38646.1; -; mRNA.
DR CCDS; CCDS14989.1; -.
DR RefSeq; NP_001272402.1; NM_001285473.1.
DR RefSeq; NP_631889.1; NM_139150.5.
DR RefSeq; XP_011236812.1; XM_011238510.1.
DR RefSeq; XP_017176083.1; XM_017320594.1.
DR AlphaFoldDB; Q8VHI4; -.
DR BioGRID; 232270; 1.
DR IntAct; Q8VHI4; 1.
DR STRING; 10090.ENSMUSP00000141169; -.
DR iPTMnet; Q8VHI4; -.
DR PhosphoSitePlus; Q8VHI4; -.
DR PaxDb; Q8VHI4; -.
DR PRIDE; Q8VHI4; -.
DR ProteomicsDB; 265531; -.
DR Antibodypedia; 34159; 199 antibodies from 28 providers.
DR DNASU; 241066; -.
DR Ensembl; ENSMUST00000180952; ENSMUSP00000137825; ENSMUSG00000026017.
DR Ensembl; ENSMUST00000187978; ENSMUSP00000141169; ENSMUSG00000026017.
DR GeneID; 241066; -.
DR KEGG; mmu:241066; -.
DR UCSC; uc007beh.2; mouse.
DR CTD; 79800; -.
DR MGI; MGI:2182269; Carf.
DR VEuPathDB; HostDB:ENSMUSG00000026017; -.
DR eggNOG; ENOG502QWYS; Eukaryota.
DR GeneTree; ENSGT00390000013916; -.
DR InParanoid; Q8VHI4; -.
DR OMA; FQHLTCM; -.
DR OrthoDB; 366551at2759; -.
DR PhylomeDB; Q8VHI4; -.
DR TreeFam; TF333279; -.
DR BioGRID-ORCS; 241066; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Carf; mouse.
DR PRO; PR:Q8VHI4; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8VHI4; protein.
DR Bgee; ENSMUSG00000026017; Expressed in spermatocyte and 213 other tissues.
DR ExpressionAtlas; Q8VHI4; baseline and differential.
DR Genevisible; Q8VHI4; MM.
DR GO; GO:0001652; C:granular component; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0071277; P:cellular response to calcium ion; ISS:UniProtKB.
DR GO; GO:0035865; P:cellular response to potassium ion; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0061400; P:positive regulation of transcription from RNA polymerase II promoter in response to calcium ion; ISS:UniProtKB.
DR GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR029309; CaRF.
DR PANTHER; PTHR14694; PTHR14694; 1.
DR Pfam; PF15299; ALS2CR8; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..689
FT /note="Calcium-responsive transcription factor"
FT /id="PRO_0000076173"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 541..609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..563
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..605
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 689 AA; 76397 MW; 34CFCD3B37D0B342 CRC64;
MEQRHSLAGN CDDGEKSERE AQGFEHRTCM DSGDPSFGQN DPPTILPITA PKTYDSLTSQ
DMPETLTDTQ ALHEEQFHLL DQNGQPIQYD LQSLGNSTAQ MTGMEQVAIP QEPFADEHSP
QDISEEKPTG VRADVLEDST SNYGLRSPAS LVLPKKAGAR LVEEPLLAPL QPLSCNTPMW
ACRLRSCEKI GDSYRGYCVS ETELESVLTF HKQQTQTVWG TRQSPSPAKP ATRLMWKSQY
VPYDGIPFVN AGSRAVVMEC QYGPRRKGFQ LKKISEQESR SCHLYKATCP ARIYIKKVQK
FPEYRVPTDP QIDRKIIRLE QEKAFTMLKK NLMDAGGVLR WYVQLPTQQA HQYHESETPG
VPLSPSPFPM SPLEEEEAIV RDENCALPSR LHPQVAHKIQ ELVSQGVGQV YAVRKQLRKF
VERELFKPDE IPERHNLSYF PTVNDIKNHI HEVQKSLRTG DVVYNSEIIP ATLQWTTDSG
NILRETVTVT FAEGNLLGEP IPSKMGTSQT QTAVSPEPLS SFPPKIFTHF QALKLQPRLS
SPDGSQALVS VDSHASSSPP GLVDTVGNAE VDNHSVLLGQ SQNPGTDTCL TQDNSTSSST
GHLPESVPNP VAEDQLLEGE DVEDAGNPEG SVNRTLLGDV QTVPIQIIDS RPVLVEESLS
KNQVKQETNE PTLSTEAKTF LDCKKISAT
