CART_MOUSE
ID CART_MOUSE Reviewed; 129 AA.
AC P56388; Q9QXZ8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Cocaine- and amphetamine-regulated transcript protein;
DE Contains:
DE RecName: Full=CART(1-52);
DE Contains:
DE RecName: Full=CART(55-102);
DE Contains:
DE RecName: Full=CART(62-102);
DE Flags: Precursor;
GN Name=Cartpt; Synonyms=Cart;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=129/SvJ;
RX PubMed=10612705; DOI=10.1016/s0006-8993(99)01907-1;
RA Adams L.D., Gong W., Vechia S.D., Hunter R.G., Kuhar M.J.;
RT "CART: from gene to function.";
RL Brain Res. 848:137-140(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-48 (ISOFORM SHORT), AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Satiety factor closely associated with the actions of leptin
CC and neuropeptide y; this anorectic peptide inhibits both normal and
CC starvation-induced feeding and completely blocks the feeding response
CC induced by neuropeptide Y and regulated by leptin in the hypothalamus.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P56388-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P56388-2; Sequence=VSP_000793;
CC -!- SIMILARITY: Belongs to the CART family. {ECO:0000305}.
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DR EMBL; AF148071; AAF24168.1; -; Genomic_DNA.
DR EMBL; BC056431; AAH56431.1; -; mRNA.
DR CCDS; CCDS36763.1; -. [P56388-1]
DR CCDS; CCDS88509.1; -. [P56388-2]
DR RefSeq; NP_038760.3; NM_013732.7. [P56388-1]
DR AlphaFoldDB; P56388; -.
DR SMR; P56388; -.
DR BioGRID; 205141; 1.
DR STRING; 10090.ENSMUSP00000022150; -.
DR iPTMnet; P56388; -.
DR PhosphoSitePlus; P56388; -.
DR PaxDb; P56388; -.
DR PeptideAtlas; P56388; -.
DR PRIDE; P56388; -.
DR ProteomicsDB; 283675; -. [P56388-1]
DR ProteomicsDB; 283676; -. [P56388-2]
DR Antibodypedia; 24184; 226 antibodies from 30 providers.
DR DNASU; 27220; -.
DR Ensembl; ENSMUST00000022150; ENSMUSP00000022150; ENSMUSG00000021647. [P56388-1]
DR Ensembl; ENSMUST00000224142; ENSMUSP00000152981; ENSMUSG00000021647. [P56388-2]
DR GeneID; 27220; -.
DR KEGG; mmu:27220; -.
DR UCSC; uc007rpw.2; mouse. [P56388-1]
DR CTD; 9607; -.
DR MGI; MGI:1351330; Cartpt.
DR VEuPathDB; HostDB:ENSMUSG00000021647; -.
DR eggNOG; ENOG502S2YU; Eukaryota.
DR GeneTree; ENSGT00390000018319; -.
DR HOGENOM; CLU_157363_1_0_1; -.
DR InParanoid; P56388; -.
DR OMA; PRTGTMQ; -.
DR PhylomeDB; P56388; -.
DR TreeFam; TF332948; -.
DR BioGRID-ORCS; 27220; 2 hits in 73 CRISPR screens.
DR PRO; PR:P56388; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P56388; protein.
DR Bgee; ENSMUSG00000021647; Expressed in arcuate nucleus of hypothalamus and 80 other tissues.
DR ExpressionAtlas; P56388; baseline and differential.
DR Genevisible; P56388; MM.
DR GO; GO:0005615; C:extracellular space; IDA:HGNC-UCL.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0005184; F:neuropeptide hormone activity; TAS:MGI.
DR GO; GO:0008343; P:adult feeding behavior; IDA:HGNC-UCL.
DR GO; GO:0001678; P:cellular glucose homeostasis; ISS:HGNC-UCL.
DR GO; GO:0009267; P:cellular response to starvation; IDA:HGNC-UCL.
DR GO; GO:0007268; P:chemical synaptic transmission; IEA:UniProtKB-KW.
DR GO; GO:0032922; P:circadian regulation of gene expression; ISS:HGNC-UCL.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:HGNC-UCL.
DR GO; GO:0032099; P:negative regulation of appetite; IMP:HGNC-UCL.
DR GO; GO:0045779; P:negative regulation of bone resorption; IGI:HGNC-UCL.
DR GO; GO:0070093; P:negative regulation of glucagon secretion; ISO:MGI.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; TAS:HGNC-UCL.
DR GO; GO:0007218; P:neuropeptide signaling pathway; TAS:MGI.
DR GO; GO:0045777; P:positive regulation of blood pressure; ISS:HGNC-UCL.
DR GO; GO:0032812; P:positive regulation of epinephrine secretion; ISS:HGNC-UCL.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IDA:HGNC-UCL.
DR GO; GO:0045860; P:positive regulation of protein kinase activity; IDA:HGNC-UCL.
DR GO; GO:0051971; P:positive regulation of transmission of nerve impulse; ISS:HGNC-UCL.
DR GO; GO:0046850; P:regulation of bone remodeling; IMP:MGI.
DR GO; GO:0050796; P:regulation of insulin secretion; ISO:MGI.
DR GO; GO:0070253; P:somatostatin secretion; ISO:MGI.
DR Gene3D; 4.10.40.30; -; 1.
DR InterPro; IPR009106; CART.
DR InterPro; IPR036722; CART_C_sf.
DR PANTHER; PTHR16655; PTHR16655; 1.
DR Pfam; PF06373; CART; 1.
DR SUPFAM; SSF64546; SSF64546; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cleavage on pair of basic residues; Disulfide bond;
KW Neuropeptide; Neurotransmitter; Phosphoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..129
FT /note="Cocaine- and amphetamine-regulated transcript
FT protein"
FT /id="PRO_0000004436"
FT PEPTIDE 28..79
FT /note="CART(1-52)"
FT /evidence="ECO:0000250"
FT /id="PRO_0000004437"
FT PEPTIDE 82..129
FT /note="CART(55-102)"
FT /evidence="ECO:0000250"
FT /id="PRO_0000004438"
FT PEPTIDE 89..129
FT /note="CART(62-102)"
FT /evidence="ECO:0000255"
FT /id="PRO_0000004439"
FT MOD_RES 41
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P49192"
FT MOD_RES 48
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT DISULFID 95..113
FT /evidence="ECO:0000250"
FT DISULFID 101..121
FT /evidence="ECO:0000250"
FT DISULFID 115..128
FT /evidence="ECO:0000250"
FT VAR_SEQ 54..66
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_000793"
FT MOD_RES P56388-2:48
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 129 AA; 14285 MW; E4CA6CE70BEE6DF2 CRC64;
MESSRLRLLP LLGAALLLLL PLLGARAQED AELQPRALDI YSAVDDASHE KELPRRQLRA
PGAMLQIEAL QEVLKKLKSK RIPIYEKKYG QVPMCDAGEQ CAVRKGARIG KLCDCPRGTS
CNSFLLKCL
