CART_THANI
ID CART_THANI Reviewed; 103 AA.
AC P0DUJ6;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 1.
DT 25-MAY-2022, entry version 5.
DE RecName: Full=Niq CART3 {ECO:0000303|PubMed:32206496};
DE AltName: Full=Cocaine- and amphetamine-regulated transcript protein-related peptide 3 {ECO:0000303|PubMed:16488069};
DE Short=CART-related peptide 3 {ECO:0000303|PubMed:16488069};
DE Contains:
DE RecName: Full=Niq CART2 {ECO:0000303|PubMed:32206496};
DE AltName: Full=Cocaine- and amphetamine-regulated transcript protein-related peptide 2 {ECO:0000303|PubMed:16488069};
DE Short=CART-related peptide 2 {ECO:0000303|PubMed:16488069};
DE Contains:
DE RecName: Full=Niq CART1 {ECO:0000303|PubMed:32206496};
DE AltName: Full=Cocaine- and amphetamine-regulated transcript protein-related peptide 1 {ECO:0000303|PubMed:16488069};
DE Short=CART-related peptide 1 {ECO:0000303|PubMed:16488069};
DE Flags: Precursor;
OS Thalassophryne nattereri (Copper Joe toadfish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Batrachoidaria; Batrachoididae; Thalassophryne.
OX NCBI_TaxID=289382;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=16488069; DOI=10.1016/j.biochi.2005.12.008;
RA Magalhaes G.S., Junqueira-de-Azevedo I.L., Lopes-Ferreira M.,
RA Lorenzini D.M., Ho P.L., Moura-da-Silva A.M.;
RT "Transcriptome analysis of expressed sequence tags from the venom glands of
RT the fish Thalassophryne nattereri.";
RL Biochimie 88:693-699(2006).
RN [2]
RP PROTEIN SEQUENCE OF 58-103, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP 3D-STRUCTURE MODELING OF NIQ CART3, AND FUNCTION.
RC TISSUE=Venom;
RX PubMed=32206496; DOI=10.1007/s13205-020-2151-4;
RA Conceicao K., de Cena G.L., da Silva V.A., de Oliveira Neto X.A.,
RA de Andrade V.M., Tada D.B., Richardson M., de Andrade S.A., Dias S.A.,
RA Castanho M.A.R.B., Lopes-Ferreira M.;
RT "Design of bioactive peptides derived from CART sequence isolated from the
RT toadfish Thalassophryne nattereri.";
RL 3 Biotech. 10:162-162(2020).
CC -!- FUNCTION: These peptides may have low toxicity to eukaryotic cells and
CC low antimicrobial activities against bacterial and fungi. It is unknown
CC whether these CART-related peptides affect feeding, as mammalian CART
CC peptides do. {ECO:0000305|PubMed:32206496}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32206496}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:32206496}.
CC -!- MASS SPECTROMETRY: [Niq CART1]: Mass=5075.28; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:32206496};
CC -!- MASS SPECTROMETRY: [Niq CART2]: Mass=5593.13; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:32206496};
CC -!- MASS SPECTROMETRY: [Niq CART3]: Mass=5724.38; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:32206496};
CC -!- MISCELLANEOUS: Two short synthetic peptides Tn CRT2 (AA 78-90) and Tn
CC CRT3 (RKGSQMGKKCECP) have antimicrobial activities against both Gram-
CC positive and -negative bacteria, as well against the yeast C.albicans.
CC {ECO:0000269|PubMed:32206496}.
CC -!- SIMILARITY: Belongs to the CART family. {ECO:0000305}.
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DR EMBL; CO816067; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; P0DUJ6; -.
DR SMR; P0DUJ6; -.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0005184; F:neuropeptide hormone activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0008343; P:adult feeding behavior; IEA:InterPro.
DR GO; GO:0009267; P:cellular response to starvation; IEA:InterPro.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IEA:InterPro.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0032099; P:negative regulation of appetite; IEA:InterPro.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IEA:InterPro.
DR Gene3D; 4.10.40.30; -; 1.
DR InterPro; IPR009106; CART.
DR InterPro; IPR036722; CART_C_sf.
DR PANTHER; PTHR16655; PTHR16655; 1.
DR Pfam; PF06373; CART; 1.
DR SUPFAM; SSF64546; SSF64546; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Fungicide; Secreted; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..52
FT /evidence="ECO:0000305"
FT /id="PRO_0000452483"
FT PEPTIDE 53..103
FT /note="Niq CART3"
FT /evidence="ECO:0000305|PubMed:32206496"
FT /id="PRO_0000452484"
FT PEPTIDE 54..103
FT /note="Niq CART2"
FT /evidence="ECO:0000305|PubMed:32206496"
FT /id="PRO_0000452485"
FT PEPTIDE 58..103
FT /note="Niq CART1"
FT /evidence="ECO:0000269|PubMed:32206496"
FT /id="PRO_0000452486"
FT DISULFID 69..87
FT /evidence="ECO:0000250|UniProtKB:Q16568"
FT DISULFID 75..95
FT /evidence="ECO:0000250|UniProtKB:Q16568"
FT DISULFID 89..102
FT /evidence="ECO:0000250|UniProtKB:Q16568"
FT VARIANT 91
FT /note="Q -> R (in Niq CART2 and Niq CART3)"
FT /evidence="ECO:0000305|PubMed:32206496"
SQ SEQUENCE 103 AA; 11602 MW; 2A4251B7A7DF4444 CRC64;
MWARAVLCAA LLSVMCLAGC TEAERDERDV QDYPYKSNRM LGALHEDVEQ LEMNRMNALE
KRYGQIRSCD VGEPCAIRKG SRMGKKCECP QGASCNRLLL KCL
