ID   Y4186_STRCO             Reviewed;         418 AA.
AC   Q9K4F4;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Uncharacterized N-acetyltransferase SCO4186 {ECO:0000255|HAMAP-Rule:MF_01812};
DE            EC=2.3.1.- {ECO:0000255|HAMAP-Rule:MF_01812};
GN   OrderedLocusNames=SCO4186; ORFNames=SCD66.23;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- SUBUNIT: Homohexamer; trimer of dimers. {ECO:0000255|HAMAP-
CC       Rule:MF_01812}.
CC   -!- SIMILARITY: Belongs to the acetyltransferase Eis family.
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DR   EMBL; AL939119; CAB94090.1; -; Genomic_DNA.
DR   RefSeq; NP_628362.1; NC_003888.3.
DR   RefSeq; WP_003974785.1; NZ_VNID01000031.1.
DR   AlphaFoldDB; Q9K4F4; -.
DR   SMR; Q9K4F4; -.
DR   STRING; 100226.SCO4186; -.
DR   PRIDE; Q9K4F4; -.
DR   GeneID; 1099626; -.
DR   KEGG; sco:SCO4186; -.
DR   PATRIC; fig|100226.15.peg.4250; -.
DR   eggNOG; COG4552; Bacteria.
DR   HOGENOM; CLU_050659_0_0_11; -.
DR   InParanoid; Q9K4F4; -.
DR   OMA; RRPWCPD; -.
DR   PhylomeDB; Q9K4F4; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0034069; F:aminoglycoside N-acetyltransferase activity; IBA:GO_Central.
DR   GO; GO:0030649; P:aminoglycoside antibiotic catabolic process; IBA:GO_Central.
DR   Gene3D; 3.30.1050.10; -; 1.
DR   HAMAP; MF_01812; Eis; 1.
DR   InterPro; IPR041380; Acetyltransf_17.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR025559; Eis_dom.
DR   InterPro; IPR000182; GNAT_dom.
DR   InterPro; IPR022902; NAcTrfase_Eis.
DR   InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR   Pfam; PF17668; Acetyltransf_17; 1.
DR   Pfam; PF13530; SCP2_2; 1.
DR   SUPFAM; SSF55718; SSF55718; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..418
FT                   /note="Uncharacterized N-acetyltransferase SCO4186"
FT                   /id="PRO_0000220265"
FT   DOMAIN          7..158
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   ACT_SITE        418
FT                   /note="Proton acceptor; via carboxylate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   BINDING         87..89
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
FT   BINDING         95..100
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01812"
SQ   SEQUENCE   418 AA;  46404 MW;  B535FC2E07ECBF84 CRC64;
     MSRRTEIDVR PITEAEFPDW NRALNTGFLR PPAVAEEQLE ARRNEFVPGR SLGAFDGDRC
     VATFRSFDQE LTAVGGGLVR ADAVSNVTVT ATHRRRGLLT RMMAQDLAAA KERGDVVATL
     IAAEYRIYGR YGFGPATSMT EWAVDVPRAG LDPRWAAPED GGRIDLVDGA DVRRLGPELH
     ERLRRAQPGA VSRTELWWRY RTGVIDWNGS PWTEPCYALY RSADGEVEGM ASYRTDDRWG
     DAKQPLNTAT VDWLLAVTPA AERALWHYLC SIDWVQTVKS GWRSPDDLLP HLLPDPRAAR
     ITTQADWLWV RILDVARALE ARTYEGQGSV VLEVVDRGGL TGGRWRLEAG PDGASCAPTT
     ESAHFVLDVG DLAALWLGDE SAVRLAALGR VREERAGAAR VVDALLRTSR RPWCPDMF