CAS1A_FRATN
ID CAS1A_FRATN Reviewed; 318 AA.
AC A0Q5Y4;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=CRISPR-associated endonuclease Cas1 1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN Name=cas1-1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=FTN_0758;
OS Francisella tularensis subsp. novicida (strain U112).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=401614;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=U112;
RX PubMed=17550600; DOI=10.1186/gb-2007-8-6-r102;
RA Rohmer L., Fong C., Abmayr S., Wasnick M., Larson Freeman T.J., Radey M.,
RA Guina T., Svensson K., Hayden H.S., Jacobs M., Gallagher L.A., Manoil C.,
RA Ernst R.K., Drees B., Buckley D., Haugen E., Bovee D., Zhou Y., Chang J.,
RA Levy R., Lim R., Gillett W., Guenthener D., Kang A., Shaffer S.A.,
RA Taylor G., Chen J., Gallis B., D'Argenio D.A., Forsman M., Olson M.V.,
RA Goodlett D.R., Kaul R., Miller S.I., Brittnacher M.J.;
RT "Comparison of Francisella tularensis genomes reveals evolutionary events
RT associated with the emergence of human pathogenic strains.";
RL Genome Biol. 8:R102.1-R102.16(2007).
RN [2]
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=U112;
RX PubMed=23584588; DOI=10.1038/nature12048;
RA Sampson T.R., Saroj S.D., Llewellyn A.C., Tzeng Y.L., Weiss D.S.;
RT "A CRISPR/Cas system mediates bacterial innate immune evasion and
RT virulence.";
RL Nature 497:254-257(2013).
RN [3]
RP ERRATUM OF PUBMED:23584588.
RX DOI=10.1038/nature12498;
RA Sampson T.R., Saroj S.D., Llewellyn A.C., Tzeng Y.L., Weiss D.S.;
RL Nature 501:602-602(2013).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- INDUCTION: In culture expression is high during early log phase (1
CC hour), decreases and then rises again in late stationary phase (16
CC hours). During infection of mouse bone marrow-derived macrophages
CC (BMDM) expression is maximal after 1 hour, when the bacteria is
CC expected to be in the phagosome. {ECO:0000269|PubMed:23584588}.
CC -!- DISRUPTION PHENOTYPE: No effect on expression of bacterial lipoprotein
CC FTN_1103. Bacteria are as virulent in mice as wild-type bacteria.
CC {ECO:0000269|PubMed:23584588}.
CC -!- MISCELLANEOUS: Part of a type II CRISPR-Cas system.
CC {ECO:0000305|PubMed:23584588}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR EMBL; CP000439; ABK89649.1; -; Genomic_DNA.
DR RefSeq; WP_003033605.1; NZ_CP009633.1.
DR AlphaFoldDB; A0Q5Y4; -.
DR SMR; A0Q5Y4; -.
DR EnsemblBacteria; ABK89649; ABK89649; FTN_0758.
DR KEGG; ftn:FTN_0758; -.
DR OMA; YYVGSFY; -.
DR OrthoDB; 1581397at2; -.
DR BioCyc; FTUL401614:G1G75-790-MON; -.
DR Proteomes; UP000000762; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.120.920; -; 1.
DR Gene3D; 3.100.10.20; -; 1.
DR HAMAP; MF_01470; Cas1; 1.
DR InterPro; IPR002729; CRISPR-assoc_Cas1.
DR InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR InterPro; IPR019855; CRISPR-assoc_Cas1_NMENI.
DR Pfam; PF01867; Cas_Cas1; 1.
DR TIGRFAMs; TIGR00287; cas1; 1.
DR TIGRFAMs; TIGR03639; cas1_NMENI; 1.
PE 2: Evidence at transcript level;
KW Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW Manganese; Metal-binding; Nuclease.
FT CHAIN 1..318
FT /note="CRISPR-associated endonuclease Cas1 1"
FT /id="PRO_0000436104"
FT BINDING 157
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 222
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 237
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ SEQUENCE 318 AA; 36558 MW; D17AF5334730C118 CRC64;
MKHLIISEYG IYLGLESGRL VVKNKDDKKY FPLNRLATLS IAKKGVSFSS DLVEQFSLRG
IKLFFLDFRG VAHSMLVGAN QHAVVQARIN QYRYIDRNAL TLSIKLIAAK IKNQRATLNY
FNKHHKSINL LNAIEELKRV AQLIKNAKTL NDVLGYEGYA ANIYFSSLAK DKFLSASFAN
REGRGSQEIA NSMLNFGYAI LSSYILNAIT NAGLEPYLGF LHQKRPGKMS LVLDLMEEYR
AWVVDRVVIK LREQYKNKQY IDTKLKSILI SEIQATIAKK YIYNGKKLKL EHIIQRQVYR
LSGEFAGEHN YKPYIFKW
