ID   CAS1A_MOOTA             Reviewed;         343 AA.
AC   Q2RL66;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1-1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Moth_0493;
OS   Moorella thermoacetica (strain ATCC 39073 / JCM 9320).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Moorella group; Moorella.
OX   NCBI_TaxID=264732;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39073 / JCM 9320;
RX   PubMed=18631365; DOI=10.1111/j.1462-2920.2008.01679.x;
RA   Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C.,
RA   Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.;
RT   "The complete genome sequence of Moorella thermoacetica (f. Clostridium
RT   thermoaceticum).";
RL   Environ. Microbiol. 10:2550-2573(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; CP000232; ABC18823.1; -; Genomic_DNA.
DR   RefSeq; WP_011392030.1; NC_007644.1.
DR   RefSeq; YP_429366.1; NC_007644.1.
DR   AlphaFoldDB; Q2RL66; -.
DR   SMR; Q2RL66; -.
DR   STRING; 264732.Moth_0493; -.
DR   PRIDE; Q2RL66; -.
DR   EnsemblBacteria; ABC18823; ABC18823; Moth_0493.
DR   KEGG; mta:Moth_0493; -.
DR   PATRIC; fig|264732.11.peg.528; -.
DR   eggNOG; COG1518; Bacteria.
DR   HOGENOM; CLU_052779_1_0_9; -.
DR   OMA; AYKLIKH; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09721; Cas1_I-C; 1.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR019856; CRISPR-assoc_Cas1_DVULG.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
DR   TIGRFAMs; TIGR03640; cas1_DVULG; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease.
FT   CHAIN           1..343
FT                   /note="CRISPR-associated endonuclease Cas1 1"
FT                   /id="PRO_0000417080"
FT   BINDING         166
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         234
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         249
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   343 AA;  39065 MW;  22CF8848D4B59C4D CRC64;
     MHILLNTLYL MTPRTLVRLD HETVKIEVEG ELKMQIPLHH LGSIVCFGDV TLTTPLIMRC
     AEDKRLIVFH DQHGRFKARV EGPVSGNVFL RHAQHEALSD SKKTTAIARN IVAGKIRNTR
     QVVLRGAREA DNEDDRKALQ ETARELAHGL DALSRSPDLE QIRGIEGNAA RHYFATLDRM
     VRVNREAFKI THRNRRPPLD RMNALLSYIY ALLLNDCLSA AEGVGLDPQI GYLHVLRSGR
     PALALDLMEE FRAILADRLA LTLVNRRQID ERDFVERPGG AVHINDNARK EIAIAYQKRK
     QEEVLHPVLN RKVPLGLVPH IQARLLARVL RGDAEEYLPF MYR