ID   CAS1A_THET8             Reviewed;         315 AA.
AC   Q53W21;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1-1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=TTHB145;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OG   Plasmid pTT27.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- MISCELLANEOUS: Probably encoded in a type III-A CRISPR locus.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP008227; BAD71941.1; -; Genomic_DNA.
DR   RefSeq; WP_011229154.1; NC_006462.1.
DR   RefSeq; YP_145384.1; NC_006462.1.
DR   AlphaFoldDB; Q53W21; -.
DR   SMR; Q53W21; -.
DR   EnsemblBacteria; BAD71941; BAD71941; BAD71941.
DR   GeneID; 3169526; -.
DR   KEGG; ttj:TTHB145; -.
DR   PATRIC; fig|300852.9.peg.2091; -.
DR   HOGENOM; CLU_052779_1_0_0; -.
DR   OMA; LYPPQNR; -.
DR   PhylomeDB; Q53W21; -.
DR   Proteomes; UP000000532; Plasmid pTT27.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Plasmid; Reference proteome.
FT   CHAIN           1..315
FT                   /note="CRISPR-associated endonuclease Cas1 1"
FT                   /id="PRO_0000417090"
FT   BINDING         144
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         208
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         223
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   315 AA;  34819 MW;  6E7A81C2D73229E2 CRC64;
     MTLHLTRQGA TLRLRQGRLL LEEEGREVAG FPARQVRSVA LWGNVRLSTP ALVFLLRQGV
     PVFFYSLEGF LHGVAGAYPD PHPAHLRAQF AAEGLPLARA FVVGKLRSAL ALLERHRLPE
     AGGVVEALAR AEGASELERL RGAEGEGSRV YFQGLARLLG PYGFGGRTRR PPRDPVNAAL
     SYGYALLLGR VLVAVRLAGL HPEVGFLHAE GRRSPALALD LMEEFRVPVV DQVVLSAFRR
     GLLTPSHAEV REGGVYLNEE GRRRLIQLFE ERLLEGVSHP LGFRKPLGET IEVQAQRLKA
     ALLGRGRYTP FYLWR