ID   CAS1B_METHJ             Reviewed;         303 AA.
AC   Q2FPW6;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Putative CRISPR-associated endonuclease Cas1 2;
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1-2 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Mhun_1196;
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- CAUTION: Missing conserved residues thought to be important for metal-
CC       binding. {ECO:0000305}.
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DR   EMBL; CP000254; ABD40943.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2FPW6; -.
DR   SMR; Q2FPW6; -.
DR   STRING; 323259.Mhun_1196; -.
DR   EnsemblBacteria; ABD40943; ABD40943; Mhun_1196.
DR   KEGG; mhu:Mhun_1196; -.
DR   eggNOG; arCOG01452; Archaea.
DR   HOGENOM; CLU_052779_2_1_2; -.
DR   OMA; NTIMFET; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..303
FT                   /note="Putative CRISPR-associated endonuclease Cas1 2"
FT                   /id="PRO_0000417102"
FT   BINDING         149
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   303 AA;  34274 MW;  746C4324E7EAEC05 CRC64;
     MGGFGAHIKS NRTEITIQHK GKITDIPIKD LSHFLLIGGH TIQTSTITSL VKEGVFISFC
     ESDGEPVGYI SPYDYSLFKE IQNLQKTAAP YSYALACANE SIKSRILAIE KYAEEIGPEI
     LFSGELDILT GYAKELENMV LIEELRRIEQ LVRDMYYEIL GRLISPTYLF KRRTSRPYLD
     PVNAIFSFGY GMLSSACTRA VIGGHLDPGH GYLNRGNQAL VQDLMNCWKP KMIDNHAIGF
     LRSGRLHQNG YERTKDRCIL HDEVIEELIH LFSKSIQEEL INTQIDVLIQ SLRGEAQFSI
     IKP