ID   CAS1B_MOOTA             Reviewed;         321 AA.
AC   Q2RHR1;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 2 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1-2 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Moth_1727;
OS   Moorella thermoacetica (strain ATCC 39073 / JCM 9320).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Moorella group; Moorella.
OX   NCBI_TaxID=264732;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39073 / JCM 9320;
RX   PubMed=18631365; DOI=10.1111/j.1462-2920.2008.01679.x;
RA   Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C.,
RA   Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.;
RT   "The complete genome sequence of Moorella thermoacetica (f. Clostridium
RT   thermoaceticum).";
RL   Environ. Microbiol. 10:2550-2573(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; CP000232; ABC20028.1; -; Genomic_DNA.
DR   RefSeq; WP_011393228.1; NC_007644.1.
DR   RefSeq; YP_430571.1; NC_007644.1.
DR   AlphaFoldDB; Q2RHR1; -.
DR   SMR; Q2RHR1; -.
DR   STRING; 264732.Moth_1727; -.
DR   EnsemblBacteria; ABC20028; ABC20028; Moth_1727.
DR   GeneID; 61290466; -.
DR   KEGG; mta:Moth_1727; -.
DR   PATRIC; fig|264732.11.peg.1873; -.
DR   eggNOG; COG1518; Bacteria.
DR   HOGENOM; CLU_052779_2_0_9; -.
DR   OMA; FIRLECY; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09722; Cas1_I-B; 1.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR019858; CRISPR-assoc_Cas1_HMARI/TNEAP.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   PANTHER; PTHR43219; PTHR43219; 1.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
DR   TIGRFAMs; TIGR03641; cas1_HMARI; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease.
FT   CHAIN           1..321
FT                   /note="CRISPR-associated endonuclease Cas1 2"
FT                   /id="PRO_0000417081"
FT   BINDING         150
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         213
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         228
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   321 AA;  38132 MW;  0F6FA63266E0AEF5 CRC64;
     MRTYYIFSSG RLRRMDNTLA LELETERRVV PVEDIDHIYC FSELDLNTRL LDFLAQKQIC
     LHFFNYYGHY SGSFIPRESQ LSGFLLVRQV EHYLDQAKRL ELARTFVEGA LHNIRRNLEK
     REYDDICSKL DEIREGIGKT ASIEELMSLE AHARKAYYDT WEEITGWEFG SRSKRPPANA
     LNALISFGNA MMYTVVLKEI YRTALNPTIS YLHEPSERRY SLALDVAEIF KPVFVDRLIF
     RLINLNMLKE THFDTNVNFV YLTEGGRKVF VKEFEETLEK TILHRKLKRN IRYKSLVRLD
     LYKLIKHLLG EEKYSPMKVW W