CAS1B_RHORT
ID CAS1B_RHORT Reviewed; 344 AA.
AC Q2RW61;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=CRISPR-associated endonuclease Cas1 2 {ECO:0000255|HAMAP-Rule:MF_01470};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN Name=cas1-2 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Rru_A0833;
OS Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
OS NCIMB 8255 / S1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Rhodospirillum.
OX NCBI_TaxID=269796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1;
RX PubMed=21886856; DOI=10.4056/sigs.1804360;
RA Munk A.C., Copeland A., Lucas S., Lapidus A., Del Rio T.G., Barry K.,
RA Detter J.C., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N.C.,
RA Mavromatis K., Richardson P., Rohde M., Goeker M., Klenk H.P., Zhang Y.,
RA Roberts G.P., Reslewic S., Schwartz D.C.;
RT "Complete genome sequence of Rhodospirillum rubrum type strain (S1).";
RL Stand. Genomic Sci. 4:293-302(2011).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR EMBL; CP000230; ABC21634.1; -; Genomic_DNA.
DR RefSeq; WP_011388588.1; NC_007643.1.
DR RefSeq; YP_425921.1; NC_007643.1.
DR AlphaFoldDB; Q2RW61; -.
DR SMR; Q2RW61; -.
DR STRING; 269796.Rru_A0833; -.
DR EnsemblBacteria; ABC21634; ABC21634; Rru_A0833.
DR KEGG; rru:Rru_A0833; -.
DR PATRIC; fig|269796.9.peg.886; -.
DR eggNOG; COG1518; Bacteria.
DR HOGENOM; CLU_052779_1_0_5; -.
DR OMA; AYKLIKH; -.
DR OrthoDB; 1581397at2; -.
DR PhylomeDB; Q2RW61; -.
DR Proteomes; UP000001929; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR CDD; cd09721; Cas1_I-C; 1.
DR Gene3D; 1.20.120.920; -; 1.
DR Gene3D; 3.100.10.20; -; 1.
DR HAMAP; MF_01470; Cas1; 1.
DR InterPro; IPR002729; CRISPR-assoc_Cas1.
DR InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR InterPro; IPR019856; CRISPR-assoc_Cas1_DVULG.
DR InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR Pfam; PF01867; Cas_Cas1; 1.
DR TIGRFAMs; TIGR00287; cas1; 1.
DR TIGRFAMs; TIGR03640; cas1_DVULG; 1.
PE 3: Inferred from homology;
KW Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW Manganese; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..344
FT /note="CRISPR-associated endonuclease Cas1 2"
FT /id="PRO_0000417084"
FT BINDING 167
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 235
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 250
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ SEQUENCE 344 AA; 37851 MW; 86997DB5BBC625E1 CRC64;
MKKLLNTVYV TTEGTGLRKD GENLVAELDG VQKGRVPLHM VGSVVVFGGT YVSPGLMGAC
AAHGITIVLL DRVGRFQARV EGPVAGNVLL RRAQYKASEA PEDIVKSLIL GKVSNQRAVL
LRALRDHGAD FPAAEALAVK DAIDRMAHIL RKVGASAEDA DHLRGAEGEA ASLYFGVFGQ
LIRSPDGDFA FRGRSRRPPL DPTNALLSFL YTLLTHDCRS ACESVGLDPA VGFLHRDRPG
RPSLALDLME ELRPVLVDRL ALSLINRRQL RATDFQRLDG GAVLLTDEAR KTVLSAWQER
KKQERRHPFL EESAPLGLVP YLQAQMLARH LRGDLDAYPP WFWK
