ID   CAS1C_METRM             Reviewed;         334 AA.
AC   D3E4V4;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=CRISPR-associated protein Cas1 3;
GN   Name=cas1-3 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=mru_1648;
OS   Methanobrevibacter ruminantium (strain ATCC 35063 / DSM 1093 / JCM 13430 /
OS   OCM 146 / M1) (Methanobacterium ruminantium).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanobrevibacter.
OX   NCBI_TaxID=634498;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35063 / DSM 1093 / JCM 13430 / OCM 146 / M1;
RX   PubMed=20126622; DOI=10.1371/journal.pone.0008926;
RA   Leahy S.C., Kelly W.J., Altermann E., Ronimus R.S., Yeoman C.J.,
RA   Pacheco D.M., Li D., Kong Z., McTavish S., Sang C., Lambie S.C.,
RA   Janssen P.H., Dey D., Attwood G.T.;
RT   "The genome sequence of the rumen methanogen Methanobrevibacter ruminantium
RT   reveals new possibilities for controlling ruminant methane emissions.";
RL   PLoS ONE 5:E8926-E8926(2010).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ADC47498.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; CP001719; ADC47498.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; D3E4V4; -.
DR   SMR; D3E4V4; -.
DR   STRING; 634498.mru_1648; -.
DR   EnsemblBacteria; ADC47498; ADC47498; mru_1648.
DR   KEGG; mru:mru_1648; -.
DR   PATRIC; fig|634498.28.peg.1649; -.
DR   eggNOG; arCOG01452; Archaea.
DR   HOGENOM; CLU_052779_0_1_2; -.
DR   Proteomes; UP000008680; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..334
FT                   /note="CRISPR-associated protein Cas1 3"
FT                   /id="PRO_0000417099"
FT   BINDING         165
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         230
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         245
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   334 AA;  38715 MW;  3675338FBE4B25B3 CRC64;
     MKILIEGYNK SIHKRDNQIL IMEKEGELEK INIKKIDDIT IIGKGSITFD ALRLISENNV
     RLMSIDYFGK INYTLEYPSN ENIFLRKQQY KTSENHKGLI IAREMIMSKM INQKSTIKTL
     NKNKKLENVK IFERNINEAI KQIGSLRFGE RTDIEKSKMK MMGIEGSASV DYWLAVNELL
     PKEIGFFSRN NRHPNDITNA SLNYAYAILA SEVNKALVIN GLDSYCGFLH FDRQKRTSLT
     FDLMEEFRQQ LVDKVVFSLV NTKQISNDDL DKRNNSISLD VRKLIIGRVL DKVNSNINYE
     GENLSYAQII DKQAKKIVNY LINGEKYTGF SLRW