ID   CAS1C_THET8             Reviewed;         332 AA.
AC   Q53VV5;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 3 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1-3 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=TTHB224;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OG   Plasmid pTT27.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; AP008227; BAD72020.1; -; Genomic_DNA.
DR   RefSeq; YP_145463.1; NC_006462.1.
DR   AlphaFoldDB; Q53VV5; -.
DR   SMR; Q53VV5; -.
DR   EnsemblBacteria; BAD72020; BAD72020; BAD72020.
DR   KEGG; ttj:TTHB224; -.
DR   PATRIC; fig|300852.9.peg.2178; -.
DR   HOGENOM; CLU_052779_1_0_0; -.
DR   OMA; YYVGSFY; -.
DR   PhylomeDB; Q53VV5; -.
DR   Proteomes; UP000000532; Plasmid pTT27.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Plasmid; Reference proteome.
FT   CHAIN           1..332
FT                   /note="CRISPR-associated endonuclease Cas1 3"
FT                   /id="PRO_0000417092"
FT   BINDING         159
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         224
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         239
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   332 AA;  37220 MW;  BF8C91677A79A11F CRC64;
     MAEGGGGVGV VYVLENEAYL SKEGGTLKVS RRAGREVLLQ KPLIAVEEIV ILGNAVVTPA
     LLKHCAQEGV GIHYLSPTGT YYAGLTRTPS KNAPARVAQF KAYLEPTWKL ALAQRFVLGK
     IRNGLVFLRR NGAEGWERLK EALLEAERAQ DEEALRGAEG RAADLYFRAF AELLPEEFAF
     GERSRRPPRD PANSLLSLAY TLLAKECESA LLVAGLDPYV GYLHEVRYGR PSLALDLMEE
     FRSVLADSVV LSLLNNRRVT LEDFDDSEGF PRLRKEAWPK FLRAWEGRLN ERIQHPLLGK
     RLAYREILLA QARILVKHLL GELPRYEPFA VR