Y4449_DROME
ID Y4449_DROME Reviewed; 424 AA.
AC Q9VCP1; Q8MSB4;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 4.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Uncharacterized protein CG4449;
GN ORFNames=CG4449;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-26; SER-32 AND SER-34, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9VCP1-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VCP1-2; Sequence=VSP_037650;
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DR EMBL; AE014297; AAF56116.2; -; Genomic_DNA.
DR EMBL; AY118950; AAM50810.1; -; mRNA.
DR RefSeq; NP_001163698.1; NM_001170227.1. [Q9VCP1-2]
DR RefSeq; NP_001262866.1; NM_001275937.1. [Q9VCP1-2]
DR RefSeq; NP_001262867.1; NM_001275938.1. [Q9VCP1-1]
DR RefSeq; NP_651134.1; NM_142877.1. [Q9VCP1-1]
DR AlphaFoldDB; Q9VCP1; -.
DR SMR; Q9VCP1; -.
DR BioGRID; 67691; 3.
DR IntAct; Q9VCP1; 1.
DR STRING; 7227.FBpp0083780; -.
DR iPTMnet; Q9VCP1; -.
DR PaxDb; Q9VCP1; -.
DR DNASU; 42748; -.
DR EnsemblMetazoa; FBtr0084388; FBpp0083780; FBgn0039065. [Q9VCP1-1]
DR EnsemblMetazoa; FBtr0301088; FBpp0290310; FBgn0039065. [Q9VCP1-2]
DR EnsemblMetazoa; FBtr0334499; FBpp0306566; FBgn0039065. [Q9VCP1-2]
DR EnsemblMetazoa; FBtr0334500; FBpp0306567; FBgn0039065. [Q9VCP1-1]
DR GeneID; 42748; -.
DR KEGG; dme:Dmel_CG4449; -.
DR UCSC; CG4449-RA; d. melanogaster. [Q9VCP1-1]
DR CTD; 42748; -.
DR FlyBase; FBgn0039065; CG4449.
DR VEuPathDB; VectorBase:FBgn0039065; -.
DR eggNOG; KOG1769; Eukaryota.
DR InParanoid; Q9VCP1; -.
DR OMA; AHMFKEV; -.
DR PhylomeDB; Q9VCP1; -.
DR BioGRID-ORCS; 42748; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42748; -.
DR PRO; PR:Q9VCP1; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039065; Expressed in secondary oocyte and 28 other tissues.
DR ExpressionAtlas; Q9VCP1; baseline and differential.
DR Genevisible; Q9VCP1; DM.
DR GO; GO:0034399; C:nuclear periphery; IDA:FlyBase.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:FlyBase.
DR InterPro; IPR022617; Rad60/SUMO-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR Pfam; PF11976; Rad60-SLD; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..424
FT /note="Uncharacterized protein CG4449"
FT /id="PRO_0000372661"
FT REGION 45..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 32
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 96
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT VAR_SEQ 186..187
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_037650"
SQ SEQUENCE 424 AA; 47744 MW; A5EF3CEA8DCCBA79 CRC64;
MSDDDCDIFS AARKRIPEIA LPKNPYNLGN DSFSKEVDYD FIEDLPKKST KTGKRKNPAA
TRKNPPKSQD DASPPKQAEH KAVEPEEDMR TERSLSPVSL LILEMEKKNG QQSDVEKHAK
ENDVGPVARR TRSSLNRSEM APPPVSPTVE VPTQTKPKKR GQKKRTSLTT TTSNSASMEV
SLPSIVGQNN EHISRRWTLA EVAARSKVVD SIDLVSAVAP RVEGFVNLDS EDEGEKEAPP
VVEEENIFDN DNPTIEVALS WLGEIQIYKL RQHQKFKHLF KELASRNGID ENDITVDMYY
NFVGPEDTPH SIGLKSFHTL TGHPTKSHNN NNVAAKIDYN PEALCRKPKK FQVKVQADKW
KHPLVIPMKK TDNFKIIFIK CAEELNCDPR TIKLFFDGDL LDPNDTPNNQ DMEGNEVIDL
KIKA
